φYeO3-12 phage tail fiber Gp17 as a promising high specific tool for recognition of Yersinia enterocolitica pathogenic serotype O:3

AMB Express

Mikael Skurnik¹  Irwin Matyjaszczyk²  Anna Pyra³  Bożena Szermer-Olearnik⁴  Jarosław Ciekot⁴  Karolina Filik⁴  Ewa Brzozowska⁴  Joanna Niedziółka-Jönson⁵  Ewa Roźniecka⁵ 

[1] Department of Bacteriology and Immunology, Faculty of Medicine, Human Microbiome Research Program, University of Helsinki, Helsinki, Finland;Division of Clinical Microbiology, Helsinki University Hospital, HUSLAB, Helsinki, Finland;Department of Mycology and Genetics, Institute of Genetics and Microbiology, University of Wrocław, 51-148, Wroclaw, Poland;Faculty of Chemistry, University of Wroclaw, 14 F. Joliot-Curie St, 50383, Wroclaw, Poland;Hirszfeld Institute of Immunology and Experimental Therapy, Polish Academy of Sciences, 12 R. Weigl St, 53114, Wroclaw, Poland;Institute of Physical Chemistry, Polish Academy of Sciences, Kasprzaka 44, 5201-224, Warsaw, Poland;

关键词: Phage;    Yersiniosis;    Tail fiber protein;    Phage adhesins;    Yersinia enterocolitica;    Diagnostic;    ELISA;   

DOI  :  10.1186/s13568-021-01341-2

来源: Springer

【 摘 要 】

Yersiniosis is an infectious zoonotic disease caused by two enteropathogenic species of Gram-negative genus Yersinia: Yersinia enterocolitica and Yersinia pseudotuberculosis. Pigs and other wild and domestic animals are reservoirs for these bacteria. Infection is usually spread to humans by ingestion of contaminated food. Yersiniosis is considered a rare disease, but recent studies indicate that it is overlooked in the diagnostic process therefore the infections with this bacterium are not often identified. Reliable diagnosis of Yersiniosis by culturing is difficult due to the slow growth of the bacteria easily overgrown by other more rapidly growing microbes unless selec-tive growth media is used. Phage adhesins recognizing bacteria in a specific manner can be an excellent diagnostic tool, es-pecially in the diagnosis of pathogens difficult for culturing. In this study, it was shown that Gp17, the tail fiber protein (TFP) of phage φYeO3-12, specifically recognizes only the pathogenic Yersinia enterocolitica serotype O:3 (YeO:3) bacteria. The ELISA test used in this work confirmed the specific interaction of this protein with YeO:3 and demonstrated a promising tool for developing the pathogen recognition method based on phage adhesins.

【 授权许可】

CC BY