Ontogeny of RORγt+ cells in the intestine of newborns and its role in the development of experimental necrotizing enterocolitis

Cell & Bioscience

Xiuhao Zhao¹  Li Lu²  Weijue Xu²  Qingfeng Sheng²  Zhibao Lv³  Nannan Sun⁴  Mingcheng Yu⁴  Yonghui Wang⁴  Lingjie Luo⁵  Ruirong Yi⁵  Jianfeng Pang⁵  Wenhua Liang⁵  Weifang Wang⁵  Feng Wang⁶ 

[1] Department of Gastrointestinal Surgery, Shanghai East Hospital, Tongji University School of Medicine, 200120, Shanghai, China;Department of General Surgery, Shanghai Children’s Hospital, Shanghai Jiao Tong University, 355 Luding Road, Putuo, Shanghai, China;Research Center of Translational Medicine, Shanghai Children’s Hospital, Shanghai Institute of Immunology, State Key Laboratory of Oncogenes and Related Genes, Shanghai Jiao Tong University School of Medicine, Shanghai, China;Department of General Surgery, Shanghai Children’s Hospital, Shanghai Jiao Tong University, 355 Luding Road, Putuo, Shanghai, China;Department of General Surgery, Shanghai Children’s Hospital, Shanghai Jiao Tong University, 355 Luding Road, Putuo, Shanghai, China;Research Center of Translational Medicine, Shanghai Children’s Hospital, Shanghai Institute of Immunology, State Key Laboratory of Oncogenes and Related Genes, Shanghai Jiao Tong University School of Medicine, Shanghai, China;Department of Medicinal Chemistry, School of Pharmacy, Fudan University, Shanghai, China;Research Center of Translational Medicine, Shanghai Children’s Hospital, Shanghai Institute of Immunology, State Key Laboratory of Oncogenes and Related Genes, Shanghai Jiao Tong University School of Medicine, Shanghai, China;Research Center of Translational Medicine, Shanghai Children’s Hospital, Shanghai Institute of Immunology, State Key Laboratory of Oncogenes and Related Genes, Shanghai Jiao Tong University School of Medicine, Shanghai, China;Shanghai Institute of Immunology and Department of Immunology and Microbiology, Shanghai Jiao Tong University School of Medicine, 280 South of Chongqing Road, Huangpu, Shanghai, China;

关键词: Neonate;    Necrotizing enterocolitis;    Intestinal immune;    RORγt;   

DOI  :  10.1186/s13578-021-00739-6

来源: Springer

【 摘 要 】

BackgroundNeonates possess an immature and plastic immune system, which is a major cause of some diseases in newborns. Necrotizing enterocolitis (NEC) is a severe and devastating intestinal disease that typically affects premature infants. However, the development of intestinal immune cells in neonates and their roles in the pathological process of NEC have not been elucidated.ResultsWe examined the ontogeny of intestinal lamina propria lymphocytes in the early life of mice and found a high percentage of RORγt+ cells (containing inflammatory Th17 and ILC3 populations) during the first week of life. Importantly, the proportion of RORγt+ cells of intestinal lamina propria further increased in both NEC mice and patients tissue than the control. Furthermore, the application of GSK805, a specific antagonist of RORγt, inhibited IL-17A release and ameliorated NEC severity.ConclusionsOur data reveal the high proportion of RORγt+ cells in newborn mice may directly contribute to the development of NEC.

【 授权许可】

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