Tandem mass tag (TMT)-based proteomic analysis of Cryptosporidium andersoni oocysts before and after excystation

Parasites & Vectors

Le-Tian Cao¹  Kai-Hui Zhang¹  Shuang-Jian Zheng¹  Dong-Fang Li¹  Zhao-Hui Cui¹  Lu-Yang Wang¹  Long-Xian Zhang¹ 

[1] College of Veterinary Medicine, Henan Agricultural University, 450000, Zhengzhou, Henan Province, People’s Republic of China;International Joint Research Laboratory for Zoonotic Diseases of Henan, 450000, Zhengzhou, Henan Province, People’s Republic of China;

关键词: TMT proteomics;    Cryptosporidium andersoni;    Oocyst;    Excystation;   

DOI  :  10.1186/s13071-021-05113-6

来源: Springer

【 摘 要 】

BackgroundCryptosporidium andersoni initiates infection by releasing sporozoites from oocysts through excystation. However, the proteins involved in excystation are unknown. Determining the proteins that participate in the excystation of C. andersoni oocysts will increase our understanding of the excystation process.MethodsCryptosporidium andersoni oocysts were collected and purified from the feces of naturally infected adult cows. Tandem mass tags (TMT), coupled with liquid chromatography–tandem mass spectrometry (LC–MS/MS) proteomic analysis, were used to investigate the proteomic expression profiles of C. andersoni oocysts before and after excystation.ResultsProteomic analysis identified a total of 1586 proteins, of which 17 were differentially expressed proteins (DEPs) upon excystation. These included 10 upregulated and seven downregulated proteins. The 17 proteins had multiple biological functions associated with control of gene expression at the level of transcription and biosynthetic and metabolic processes. Quantitative real-time RT-PCR of eight selected genes validated the proteomic data.ConclusionsThis study provides information on the protein composition of C. andersoni oocysts as well as possible excystation factors. The data may be useful in identifying genes for diagnosis, vaccine development, and immunotherapy for Cryptosporidium.Graphical Abstract

【 授权许可】

CC BY