Growth, respiratory activity and chlorpyrifos biodegradation in cultures of Azotobacter vinelandii ATCC 12837

AMB Express

Pedro de Jesús Bastidas Bastidas¹  Holjes Salgado-Lugo²  Carlos Peña²  Carmen Martínez Valenzuela³  José Belisario Leyva Morales³  Luis Daniel Ortega Martínez⁴  Beatriz Pérez-Armendáriz⁴  Victoria Conde-Avila⁴  Octavio Loera⁵ 

[1] Centro de Investigación en Alimentación y Desarrollo, A.C. (Unidad Culiacán), Carretera a El dorado km. 5.5, Campo el Diez, 80129, Culiacán, Sinaloa, México;Departamento de Ingeniería Celular y Biocatálisis, Instituto de Biotecnología, Universidad Nacional Autónoma de México, Apdo. Post. 510-3, 62250, Cuernavaca, Morelos, México;Doctorado en Sustentabilidad, Universidad Autónoma de Occidente, Unidad Guasave. Avenida Universidad S/N, Fraccionamiento Villa Universidad, 81048, Guasave, Sinaloa, México;Facultad de Biotecnología, Universidad Popular Autónoma del Estado de Puebla, México, 13 Poniente No. 1927 Col. Barrio de Santiago, 72410, Puebla, Pue, México;Universidad Autónoma Metropolitana-Unidad Iztapalapa, Av. San Rafael Atlixco 186, 09340, Mexico City, CDMX, México;

关键词: Oxygen consumption rate;    Pesticide degradation;    Respiratory quotient;    Rhizobacteria;   

DOI  :  10.1186/s13568-021-01339-w

来源: Springer

【 摘 要 】

This study aimed to evaluate the growth, respiratory activity, and biodegradation of chlorpyrifos in cultures of Azotobacter vinelandii ATCC 12837. A strategy based on the modification of culture media and aeration conditions was carried out to increase the cell concentration of A. vinelandii, in order to favor and determine its tolerance to chlorpyrifos and its degradation ability. The culture in shaken flasks, using sucrose as a carbon source, significantly improved the growth compared to media with mannitol. When the strain was cultivated under oxygen-limited (5.5, 11.25 mmol L−1 h−1) and no-oxygen-limited conditions (22 mmol L−1 h−1), the growth parameters were not affected. In cultures in a liquid medium with chlorpyrifos, the bacteria tolerated a high pesticide concentration (500 ppm) and the growth parameters were improved even under conditions with a reduced carbon source (sucrose 2 g L−1). The strain degraded 99.6% of chlorpyrifos at 60 h of cultivation, in co-metabolism with sucrose; notably, A. vinelandii ATCC 12837 reduced by 50% the initial pesticide concentration in only 6 h (DT50).Graphical Abstract

【 授权许可】

CC BY