Reference ranges of T lymphocyte subsets by single-platform among healthy population in southwest China

BMC Immunology

Huixiu Zhong¹  Liangjun Zhang¹  Weiping Liu¹  Qian Niu²  Bin Wei²  Qiang Miao²  Yi Li²  Yangjuan Bai²  Lin Yan²  Weihua Feng²  Ying Guo³ 

[1] Department of Laboratory Medicine, Zigong First People’s Hospital, Zigong, Sichuan Province, China;Department of Laboratory Medicine/Clinical Research Center of Laboratory Medicine, West China Hospital, Sichuan University, 37 Guo Xue Xiang, 610041, Chengdu, Sichuan Province, China;West China Second University Hospital/West China Women’s and Children’s Hospital, Key Laboratory of Birth Defects and Related Diseases of Women and Children, Sichuan University, No. 20, Section 3, Renmin South Road, Wuhou District, 610041, Chengdu, Sichuan Province, China;

关键词: Reference ranges;    T lymphocyte subsets;    Single-platform;    Southwest China;    Indirect method;   

DOI  :  10.1186/s12865-021-00474-0

来源: Springer

【 摘 要 】

BackgroundAppropriate reference ranges of T lymphocyte subsets are essential for immune status evaluation of patients with immunological diseases. We aim to establish the age- and sex-related reference intervals of T lymphocyte subsets by single-platform for the southwest China population using the indirect method with the data resulting from 53,822 cases of periodic health examination individuals in the Laboratory Information System (LIS) of West China Hospital from 2018 to 2020.MethodsWe used the Box-Cox conversion combined with the Tukey method to normalize the data and eliminate the outliers, and the nonparametric method to estimate the 95% distribution reference intervals.ResultsWe initially established the reference ranges of T lymphocyte subsets by single-platform among healthy population in southwest China by indirect method (See text for details). Using the standard normal deviate test (z-test) suggested by Harris and Boyd according to CLSI EP28-A3C, which is more scientific, we found the reference ranges of T lymphocyte subsets should be differentiated by ages and genders since the reference ranges of T lymphocyte subsets by single-platform in different ages and genders are significantly different.ConclusionsWe further demonstrated the absolute count of CD3 + T cell, CD3 + CD4 + T cell, CD3 + CD8 + T cell decreased with aging, which is more marked in men and CD3 + CD8 + T cell count, and the obtained reference intervals were superior to the reference intervals derived from the reagent specification currently in use.

【 授权许可】

CC BY