Extracellular vesicles carrying miRNA-181b-5p affects the malignant progression of acute lymphoblastic leukemia

Journal of Translational Medicine

Wei Yang¹  Ying Yang¹  Huihan Wang¹  Wei Yan¹  Li Song²  Liang Hu³ 

[1] Department of Hematology, Shengjing Hospital of China Medical University, 110000, Shenyang, People’s Republic of China;National Drug Clinical Trial Institute Office, Qingdao Women and Children’s Hospital, Qingdao, China;Shanghai Engineering Research Center of Pharmaceutical Translation, Shanghai, China;

关键词: miRNA-181b-5p;    ALL;    Extracellular vesicles;    Proliferation;    Cell cycle;   

DOI  :  10.1186/s12967-021-03174-w

来源: Springer

【 摘 要 】

ObjectiveTo investigate how serum extracellular vesicles (EVs)-carried miRNA-181b-5p affected the proliferation, cell cycle and apoptosis of acute lymphoblastic leukemia (ALL) cells.MethodsDifferentially expressed miRNAs related to ALL were screened by bioinformatics analysis, and the localization of target miRNA was searched by its expression. qRT-PCR was adopted to confirm the expression of miRNA-181b-5p. Flow cytometry and fluorescence microscopy were applied to evaluate EVs internalization. MTT assay was employed to verify the proliferation of ALL cells. Cell cycle and apoptosis were analyzed by flow cytometry. Transwell assay was applied to evaluate migration and invasion abilities.ResultsHigh expression of miRNA-181b-5p was proved in ALL cell lines, and miRNA-181b-5p enriched in the exosomes and vesicles of blood cells. In the meantime, it was found that EVs carrying miRNA-181b-5p could be internalized by ALL cells and thus the expression of miRNA-181b-5p was up-regulated. Cell function assays showed that the proliferation, migration, invasion abilities of ALL cell lines were promoted in miRNA-181b-5p mimic group or the group co-culturing ALL-derived EVs and BALL-1 cell lines. The percentage of cells in G0/G1 phase was reduced and cell apoptosis was also inhibited.ConclusionmiRNA-181b-5p carried by EVs in peripheral blood of ALL patients can enter ALL cells and thus promote the malignancy of ALL cells.

【 授权许可】

CC BY