Cocktail biosynthesis of triacylglycerol by rational modulation of diacylglycerol acyltransferases in industrial oleaginous Aurantiochytrium

Biotechnology for Biofuels

Weijian Wan¹  Huidan Zhang²  Sen Wang²  Huan Liu²  Xiaojin Song³  Qiu Cui⁴  Chuanzeng Lan⁵  Zhuojun Wang⁵  Yang Hu⁶ 

[1] CAS Key Laboratory of Biofuels, Shandong Provincial Key Laboratory of Energy Genetics, Shandong Engineering Laboratory of Single Cell Oil, Qingdao Engineering Laboratory of Single Cell Oil, Qingdao Institute of Bioenergy and Bioprocess Technology, Chinese Academy of Sciences, No.189 Songling Road, Laoshan District, 266101, Qingdao, Shandong, China;CAS Key Laboratory of Biofuels, Shandong Provincial Key Laboratory of Energy Genetics, Shandong Engineering Laboratory of Single Cell Oil, Qingdao Engineering Laboratory of Single Cell Oil, Qingdao Institute of Bioenergy and Bioprocess Technology, Chinese Academy of Sciences, No.189 Songling Road, Laoshan District, 266101, Qingdao, Shandong, China;Shandong Energy Institute, 266101, Qingdao, Shandong, China;Qingdao New Energy Shandong Laboratory, 266101, Qingdao, Shandong, China;CAS Key Laboratory of Biofuels, Shandong Provincial Key Laboratory of Energy Genetics, Shandong Engineering Laboratory of Single Cell Oil, Qingdao Engineering Laboratory of Single Cell Oil, Qingdao Institute of Bioenergy and Bioprocess Technology, Chinese Academy of Sciences, No.189 Songling Road, Laoshan District, 266101, Qingdao, Shandong, China;Shandong Energy Institute, 266101, Qingdao, Shandong, China;Qingdao New Energy Shandong Laboratory, 266101, Qingdao, Shandong, China;Center for Ocean Mega-Science, Chinese Academy of Sciences, 266101, Qingdao, Shandong, China;University of Chinese Academy of Sciences, 100049, Beijing, China;CAS Key Laboratory of Biofuels, Shandong Provincial Key Laboratory of Energy Genetics, Shandong Engineering Laboratory of Single Cell Oil, Qingdao Engineering Laboratory of Single Cell Oil, Qingdao Institute of Bioenergy and Bioprocess Technology, Chinese Academy of Sciences, No.189 Songling Road, Laoshan District, 266101, Qingdao, Shandong, China;Shandong Energy Institute, 266101, Qingdao, Shandong, China;Qingdao New Energy Shandong Laboratory, 266101, Qingdao, Shandong, China;University of Chinese Academy of Sciences, 100049, Beijing, China;CAS Key Laboratory of Biofuels, Shandong Provincial Key Laboratory of Energy Genetics, Shandong Engineering Laboratory of Single Cell Oil, Qingdao Engineering Laboratory of Single Cell Oil, Qingdao Institute of Bioenergy and Bioprocess Technology, Chinese Academy of Sciences, No.189 Songling Road, Laoshan District, 266101, Qingdao, Shandong, China;University of Chinese Academy of Sciences, 100049, Beijing, China;Faculty of Science, University of Calgary, T2N 1N4, Calgary, AB, Canada;

关键词: Aurantiochytrium;    Diacylglycerol acyltransferase;    Polyunsaturated fatty acid;    Saturated fatty acid;    Thraustochytrid;    Triacylglycerol;   

DOI  :  10.1186/s13068-021-02096-5

来源: Springer

【 摘 要 】

BackgroundTriacylglycerol (TAG) is an important storage lipid in organisms, depending on the degree of unsaturation of fatty acid molecules attached to glycerol; it is usually used as the feedstock for nutrition or biodiesel. However, the mechanism of assembly of saturated fatty acids (SFAs) or polyunsaturated fatty acids (PUFAs) into TAGs remains unclear for industrial oleaginous microorganism.ResultsDiacylglycerol acyltransferase (DGAT) is a key enzyme for TAG synthesis. Hence, ex vivo (in yeast), and in vivo functions of four DGAT2s (DGAT2A, DGAT2B, DGAT2C, and DGAT2D) in industrial oleaginous thraustochytrid Aurantiochytrium sp. SD116 were analyzed. Results revealed that DGAT2C was mainly responsible for connecting PUFA to the sn-3 position of TAG molecules. However, DGAT2A and DGAT2D target SFA and/or MUFA.ConclusionsThere are two specific TAG assembly routes in Aurantiochytrium. The “saturated fatty acid (SFA) TAG lane” primarily produces SFA-TAGs mainly mediated by DGAT2D whose function is complemented by DGAT2A. And, the “polyunsaturated fatty acid (PUFA) TAG lane” primarily produces PUFA-TAGs via DGAT2C. In this study, we demonstrated the functional distribution pattern of four DGAT2s in oleaginous thraustochytrid Aurantiochytrium, and provided a promising target to rationally design TAG molecular with the desired characteristics.

【 授权许可】

CC BY