Floral organ-specific proteome profiling of the floral ornamental orchid (Cymbidium goeringii) reveals candidate proteins related to floral organ development

Botanical Studies

Zihan Xu¹  Yue Chen²  Chongbo Sun²  Qi Shen³ 

[1] College of Landscape and Architecture, Nanjing Forestry University, Nanjing, Jiangsu, China;Institute of Horticulture, Zhejiang Academy of Agriculture Science, Hangzhou, Zhejiang, China;Plant Protection and Microbiology, Zhejiang Academy of Agricultural Science, Hangzhou, Zhejiang, China;

关键词: Cymbidium;    Floral organ;    Proteomic analysis;    Tandem Mass Tag;    Transcription factor;   

DOI  :  10.1186/s40529-021-00330-9

来源: Springer

【 摘 要 】

BackgroundCymbidium goeringii, belonging to the Orchidaceae family, is an important ornamental plant with striking petals and lips. Extremely diversified floral patterns and morphologies make C. goeringii good research material to examine floral development of orchids. However, no floral organ-specific protein has been identified yet. To screen floral development associated proteins, four proteomes from petal (PE), lip (LI), gynostemium (GY), and sepal (SE) were analyzed using Tandem Mass Tag-based proteomic analysis.ResultsA total of 6626 unique peptides encoding 2331 proteins were identified in our study. Proteins in several primary metabolic pathways, including amino acid metabolism, energy metabolism, and lipid metabolism, were identified as differentially expressed proteins. Interestingly, most of the energy metabolism-related proteins highly expressed in SE, indicating that SE is an important photosynthetic organ of C. goeringii flower. Furthermore, a number of phytohormone-related proteins and transcription factors (TFs) were identified in C. goeringii flowers. Expression analysis showed that 1-aminocyclopropane-1-carboxylate oxidase highly expressed in GY, IAA-amino acid hydrolase ILR1-like 4 and gibberellin receptor 1 C greatly expressed in LI, and auxin-binding protein ABP20 significantly expressed in SE, suggesting a significant role of hormones in the regulation of flower morphogenesis and development. For TFs, GY-highly expressed bHLH13, PE-highly expressed WRKY33, and GY-highly expressed VIP1, were identified.ConclusionsMining of floral organ differential expressed enzymes and TFs helps us to excavate candidate proteins related to floral organ development and to accelerate the breeding of Cymbidium plants.

【 授权许可】

CC BY