Long terminal repeats (LTR) and transcription factors regulate PHRE1 and PHRE2 activity in Moso bamboo under heat stress

BMC Plant Biology

Qiang Wei¹  Kunnummal Kurungara Vinod²  Ruslan Kalendar³  Pradeep K. Papolu⁴  Kim Yrjala⁴  Long-Hai Zou⁴  Muthusamy Ramakrishnan⁵  Mingbing Zhou⁶ 

[1]Co-Innovation Center for Sustainable Forestry in Southern China, Nanjing Forestry University, 210037, Nanjing, Jiangsu, China

[2]Bamboo Research Institute, Nanjing Forestry University, 210037, Nanjing, Jiangsu, China

[3]Division of Genetics, ICAR - Indian Agricultural Research Institute, 110012, New Delhi, India

[4]Helsinki Institute of Life Science HiLIFE, Biocenter 3, Viikinkaari 1, FI-00014 University of Helsinki, Helsinki, Finland

[5]State Key Laboratory of Subtropical Silviculture, Zhejiang A&F University, Lin’an, 311300, Hangzhou, Zhejiang, China

[6]State Key Laboratory of Subtropical Silviculture, Zhejiang A&F University, Lin’an, 311300, Hangzhou, Zhejiang, China

[7]Co-Innovation Center for Sustainable Forestry in Southern China, Nanjing Forestry University, 210037, Nanjing, Jiangsu, China

[8]Bamboo Research Institute, Nanjing Forestry University, 210037, Nanjing, Jiangsu, China

[9]State Key Laboratory of Subtropical Silviculture, Zhejiang A&F University, Lin’an, 311300, Hangzhou, Zhejiang, China

[10]Zhejiang Provincial Collaborative Innovation Centre for Bamboo Resources and High-efficiency Utilization, Zhejiang A&F University, 311300, Hangzhou, Zhejiang, China

关键词: Moso bamboo;    LTR-retrotransposons;    Transposable elements;    PHRE1;    siRNA;    Heat stress;    Transposition;   

DOI  :  10.1186/s12870-021-03339-1

来源: Springer

【 摘 要 】

BackgroundLTR retrotransposons play a significant role in plant growth, genome evolution, and environmental stress response, but their regulatory response to heat stress remains unclear. We have investigated the activities of two LTR retrotransposons, PHRE1 and PHRE2, of moso bamboo (Phyllostachys edulis) in response to heat stress.ResultsThe differential overexpression of PHRE1 and PHRE2 with or without CaMV35s promoter showed enhanced expression under heat stress in transgenic plants. The transcriptional activity studies showed an increase in transposition activity and copy number among moso bamboo wild type and Arabidopsis transgenic plants under heat stress. Comparison of promoter activity in transgenic plants indicated that 5’LTR promoter activity was higher than CaMV35s promoter. Additionally, yeast one-hybrid (Y1H) system and in planta biomolecular fluorescence complementation (BiFC) assay revealed interactions of heat-dependent transcription factors (TFs) with 5’LTR sequence and direct interactions of TFs with pol and gag.ConclusionsOur results conclude that the 5’LTR acts as a promoter and could regulate the LTR retrotransposons in moso bamboo under heat stress.

【 授权许可】

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