BVES is a novel interactor of ANO5 and regulates myoblast differentiation

Cell & Bioscience

Zhiheng Chen¹  Zuocheng Yang¹  Lingling Zhao¹  Haiwen Li²  Yandi Gao²  Renzhi Han²  Li Xu²  Shuliang Guo²  Yuanbojiao Zuo³ 

[1] Department of Pediatrics, The Third Xiangya Hospital, Central South University, Changsha, China;Division of Cardiac Surgery, Department of Surgery, Davis Heart and Lung Research Institute, The Ohio State University Wexner Medical Center, 43210, Columbus, OH, USA;Division of Cardiac Surgery, Department of Surgery, Davis Heart and Lung Research Institute, The Ohio State University Wexner Medical Center, 43210, Columbus, OH, USA;Department of Pediatrics, The Third Xiangya Hospital, Central South University, Changsha, China;

关键词: ANO5;    BioID2;    BVES;    Muscle differentiation;    Muscular dystrophy;    Proximity labeling;   

DOI  :  10.1186/s13578-021-00735-w

来源: Springer

【 摘 要 】

BackgroundAnoctamin 5 (ANO5) is a membrane protein belonging to the TMEM16/Anoctamin family and its deficiency leads to the development of limb girdle muscular dystrophy R12 (LGMDR12). However, little has been known about the interactome of ANO5 and its cellular functions.ResultsIn this study, we exploited a proximal labeling approach to identify the interacting proteins of ANO5 in C2C12 myoblasts stably expressing ANO5 tagged with BioID2. Mass spectrometry identified 41 unique proteins including BVES and POPDC3 specifically from ANO5-BioID2 samples, but not from BioID2 fused with ANO6 or MG53. The interaction between ANO5 and BVES was further confirmed by co-immunoprecipitation (Co-IP), and the N-terminus of ANO5 mediated the interaction with the C-terminus of BVES. ANO5 and BVES were co-localized in muscle cells and enriched at the endoplasmic reticulum (ER) membrane. Genome editing-mediated ANO5 or BVES disruption significantly suppressed C2C12 myoblast differentiation with little impact on proliferation.ConclusionsTaken together, these data suggest that BVES is a novel interacting protein of ANO5, involved in regulation of muscle differentiation.

【 授权许可】

CC BY