Metformin attenuates sepsis-induced neuronal injury and cognitive impairment

BMC Neuroscience

Cuiping Guo¹  Xiaochan Xiao¹  Chenliang Zhou¹  Zhenghui Qin² 

[1] Department of Critical Care Medicine, Renmin Hospital of Wuhan University, Wuhan, China;Department of Critical Care Medicine, Tianyou Hospital, Wuhan University of Science and Technology, Wuhan, China;

关键词: Sepsis;    Cecum ligation and puncture;    Metformin;    Cognitive impairment;    Inflammatory factors;   

DOI  :  10.1186/s12868-021-00683-8

来源: Springer

【 摘 要 】

BackgroundSepsis is considered to be a high-risk factor for cognitive impairment in the brain. The purpose of our study is to explore whether sepsis causes cognitive impairment and try to evaluate the underlying mechanisms and intervention measures.MethodsHere, we used cecum ligation and puncture (CLP) to simulate sepsis. Open field, Novel Objective Recognition, and Morris Water Maze Test were used to detect cognitive function, long-term potentiation was used to assess of synaptic plasticity, and molecular biological technics were used to assess synaptic proteins, ELISA kits were used to detect inflammatory factors. Metformin was injected into the lateral ventricle of SD rats, and we evaluated whether metformin alleviated CLP-mediated cognitive impairment using behavioral, electrophysiological and molecular biological technology experiments.ResultsHere we report hippocampal-dependent cognitive deficits and synaptic dysfunction induced by the CLP, accompanied by a significant increase in inflammatory factors. At the same time, metformin was able to improve cognitive impairment induced by CLP in adult male rats.ConclusionThese findings highlight a novel pathogenic mechanism of sepsis-related cognitive impairment through activation of inflammatory factors, and these are blocked by metformin to attenuate sepsis-induced neuronal injury and cognitive impairment.

【 授权许可】

CC BY