Ferulic acid ameliorates lipopolysaccharide-induced tracheal injury via cGMP/PKGII signaling pathway

Respiratory Research

Yong Cui¹  Xiaoyong Xie²  Hongguang Nie³  Yapeng Hou³  Tong Yu³  Yan Ding³  Aixin Han³ 

[1] Department of Anesthesiology, the First Hospital of China Medical University, 110001, Shenyang, China;Department of Anesthesiology, the First Hospital of China Medical University, 110001, Shenyang, China;Department of Stem Cells and Regenerative Medicine, College of Basic Medical Science, China Medical University, 110122, Shenyang, China;Department of Stem Cells and Regenerative Medicine, College of Basic Medical Science, China Medical University, 110122, Shenyang, China;

关键词: Ferulic acid;    Tracheal injury;    Epithelial sodium channel;    cGMP/PKGII signaling pathway;   

DOI  :  10.1186/s12931-021-01897-4

来源: Springer

【 摘 要 】

BackgroundTracheal injury is a common clinical condition that still lacks an effective therapy at present. Stimulation of epithelial sodium channel (ENaC) increases Na+ transport, which is a driving force to keep tracheal mucosa free edema fluid during tracheal injury. Ferulic acid (FA) has been proved to be effective in many respiratory diseases through exerting anti-oxidant, anti-inflammatory, and anti-thrombotic effects. However, these studies rarely involve the level of ion transport, especially ENaC.MethodsC57BL/J male mice were treated intraperitoneally with normal saline or FA (100 mg/kg) 12 h before, and 12 h after intratracheal administration of lipopolysaccharide (LPS, 5 mg/kg), respectively. The effects of FA on tracheal injury were not only assessed through HE staining, immunofluorescence assay, and protein/mRNA expressions of ENaC located on tracheas, but also evaluated by the function of ENaC in mouse tracheal epithelial cells (MTECs). Besides, to explore the detailed mechanism about FA involved in LPS-induced tracheal injury, the content of cyclic guanosine monophosphate (cGMP) was measured, and Rp-cGMP (cGMP inhibitor) or cGMP-dependent protein kinase II (PKGII)-siRNA (siPKGII) were applied in primary MTECs, respectively.ResultsHistological examination results demonstrated that tracheal injury was obviously attenuated by pretreatment of FA. Meanwhile, FA could reverse LPS-induced reduction of both protein/mRNA expressions and ENaC activity. ELISA assay verified cGMP content was increased by FA, and administration of Rp-cGMP or transfection of siPKGII could reverse the FA up-regulated ENaC protein expression in MTECs.ConclusionsFerulic acid can attenuate LPS-induced tracheal injury through up-regulation of ENaC at least partially via the cGMP/PKGII pathway, which may provide a promising new direction for preventive and therapeutic strategy in tracheal injury.

【 授权许可】

CC BY