Insecticide susceptibility status and knockdown resistance (kdr) mutation in Aedes albopictus in China

Parasites & Vectors

Jiachun Zhang¹  Song He¹  Xuli Xin¹  Yong Wei²  Ke Hu³  Xueli Zheng³  Daibin Zhong⁴  Guofa Zhou⁴ 

[1] Clinical Laboratory, Shenzhen Qianhai Shekou Free Trade Zone Hospital, Shenzhen, China;Clinical Laboratory, Shenzhen Qianhai Shekou Free Trade Zone Hospital, Shenzhen, China;Department of Pathogen Biology, School of Public Health, Southern Medical University, Guangzhou, China;Department of Pathogen Biology, School of Public Health, Southern Medical University, Guangzhou, China;Program in Public Health, College of Health Sciences, University of California, Irvine, CA, USA;

关键词: Aedes albopictus;    Deltamethrin;    Resistance;    kdr;   

DOI  :  10.1186/s13071-021-05095-5

来源: Springer

【 摘 要 】

BackgroundAedes (Stegomyia) albopictus (Skuse, 1894) is the main vector of dengue virus in China. The resistance to insecticides is a huge obstacle for the control of this species, and determining its resistance status and mechanisms in China is essential for the implementation of vector management strategies.MethodsWe have investigated the larval and adult resistance status of Ae. albopictus to deltamethrin in eight field populations in China. Mutations at the voltage-gated sodium channel gene, related to the knockdown resistance (kdr) effect, were detected by sequencing of PCR products. The eight field populations were examined for pyrethroid resistance using the World Health Organization standard bioassays, and the association between the mutations and phenotypic resistance was tested.ResultsThe eight field populations of larvae of Ae. albopictus in China exhibited high resistance to deltamethrin; the RR50 values ranged from 12 (ZJ) to 44 (GZ). Adult bioassay revealed that Ae. albopictus populations were resistant to deltamethrin (mortality rate < 90%), except ZJ population (probably resistant, mortality rate = 93.5%). Long knockdown time in the field populations was consistent with low mortality rates in adult bioassay. F1534S mutation showed increased protection against deltamethrin in all populations except BJ and SJZ populations, whereas I1532T mutation showed increased protection against deltamethrin in only BJ population.ConclusionThere were different degrees of resistance to deltamethrin in field Ae. albopictus populations in China. The longest knockdown time and lowest mortality rate observed in Ae. albopictus population in Guangzhou indicate the severity of high resistance to deltamethrin. The patchy distribution of deltamethrin resistance and kdr mutations in Ae. albopictus mosquitoes suggests the necessity for resistance management and developing counter measures to mitigate the spread of resistance.Graphical Abstract

【 授权许可】

CC BY