期刊论文详细信息
Journal of Orthopaedic Surgery and Research | |
Circ-ATAD1 is overexpressed in osteosarcoma (OS) and suppresses the maturation of miR-154-5p to increase cell invasion and migration | |
Peng Yang1  Shibang Lin2  Jihui Zhou2  Haizhou Huang2  Li Xu3  | |
[1]Department of Hand and Foot Surgery, The Eighth People’s Hospital of Qingdao, 266100, Qingdao City, Shandong Province, People’s Republic of China | |
[2]Department of Traumatic Orthopedics, Maoming People’s Hospital, No. 101 Weimin Road, 525000, Maoming City, Guangdong Province, People’s Republic of China | |
[3]The 9th Recuperation Department of Qingdao Special Servicemen Recuperation Center of PLA NAVY, 266071, Qingdao City, Shandong Province, People’s Republic of China | |
关键词: Osteosarcoma; Circ-ATAD1; miR-154-5p; Maturation; Migration; Invasion; | |
DOI : 10.1186/s13018-021-02809-4 | |
来源: Springer | |
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【 摘 要 】
BackgroundCirc-ATAD1 plays an oncogenic role in gastric cancer. However, its roles in other cancers are unclear. We aimed to analyze the role of circ-ATAD1 in osteosarcoma (OS).MethodsThe expression levels of circ-ATAD1, mature miR-154-5p, and premature miR-154-5p in paired OS and non-tumor tissues from 56 OS patients were determined using RT-qPCR. Nuclear fractionation assay was performed to analyze the subcellular location of circ-ATAD1. The interaction between circ-ATAD1 and premature miR-154-5p was analyzed using RNA pull-down assay. The role of circ-ATAD1 in regulating miR-154-5p maturation was analyzed using RT-qPCR in cells with overexpression. Transwell assays were performed to analyze the roles of circ-ATAD1 and miR-154-5p in regulating OS cell invasion and migration.ResultsCirc-ATAD1 was overexpressed in OS compared to non-tumor tissues and was detected in the nuclei of OS cells. Mature miR-154-5p, but not premature miR-154-5p, was downregulated in OS tissues compared to non-tumor tissues and was inversely correlated with circ-ATAD1. In OS cells, circ-ATAD1 overexpression decreased the expression of mature miR-154-5p, but not premature miR-154-5p. Transwell assay analysis showed that circ-ATAD1 overexpression increased cell invasion and migration, and mature miR-154-5p overexpression suppressed these cell behaviors. In addition, circ-ATAD1 overexpression reduced the effects of mature miR-154-5p overexpression on cell behaviors.ConclusionsCirc-ATAD1 is overexpressed in OS and suppresses miR-154-5p maturation to increase cell invasion and migration.【 授权许可】
CC BY
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