The key regulator LcERF056 enhances salt tolerance by modulating reactive oxygen species-related genes in Lotus corniculatus

BMC Plant Biology

Yanmin Wu¹  Yixiong Tang¹  Zhanmin Sun¹  Dan Wang²  Yuandong Xu³  Xinxu Hu⁴  Junbo Xiong⁵  Lizhen Hu⁶ 

[1] Biotechnology Research Institute, Chinese Academy of Agricultural Sciences, Beijing, China;Biotechnology Research Institute, Chinese Academy of Agricultural Sciences, Beijing, China;Key Laboratory of Southwest China Wildlife Resources Conservation (Ministry of Education), China West Normal University, Nanchong, China;ChongQing Academy of Animal Sciences, Chongqing, China;College of Animal Science and Technology, Hunan Agricultural University, Changsha, China;Huanshan group, Qingdao, China;Hubei Key Laboratory of Animal Embryo and Molecular Breeding, Institute of Animal and Veterinary Science, Hubei Academy of Agricultural Science, Wuhan, China;Institute of Animal and Veterinary Science, Jiangxi Academy of Agricultural Sciences, Nanchang, China;

关键词: ERF transcription factor;    Salt stress;    Regulation mechanism;    Lotus corniculatus;   

DOI  :  10.1186/s12870-021-03336-4

来源: Springer

【 摘 要 】

BackgroundThe APETALA2/ethylene response factor (AP2/ERF) family are important regulatory factors involved in plants’ response to environmental stimuli. However, their roles in salt tolerance in Lotus corniculatus remain unclear.ResultsHere, the key salt-responsive transcription factor LcERF056 was cloned and characterised. LcERF056 belonging to the B3–1 (IX) subfamily of ERFs was considerably upregulated by salt treatment. LcERF056-fused GFP was exclusively localised to nuclei. Furthermore, LcERF056- overexpression (OE) transgenic Arabidopsis and L. corniculatus lines exhibited significantly high tolerance to salt treatment compared with wild-type (WT) or RNA interference expression (RNAi) transgenic lines at the phenotypic and physiological levels. Transcriptome analysis of OE, RNAi, and WT lines showed that LcERF056 regulated the downstream genes involved in several metabolic pathways. Chromatin immunoprecipitation-quantitative polymerase chain reaction (ChIP-qPCR) and yeast one-hybrid (Y1H) assay demonstrated that LcERF056 could bind to cis-element GCC box or DRE of reactive oxygen species (ROS)-related genes such as lipid-transfer protein, peroxidase and ribosomal protein.ConclusionOur results suggested that the key regulator LcERF056 plays important roles in salt tolerance in L. corniculatus by modulating ROS-related genes. Therefore, it may be a useful target for engineering salt-tolerant L. corniculatus or other crops.

【 授权许可】

CC BY