A comprehensive comparison between camelid nanobodies and single chain variable fragments

Biomarker Research

Yasaman Asaadi¹  Fatemeh Fazlollahi Jouneghani²  Sara Janani²  Fatemeh Rahbarizadeh³ 

[1] Department of Biotechnology, College of Science, University of Tehran, Tehran, Iran;Department of Cell & Molecular Biology, Faculty of Life Sciences and Biotechnology, Shahid Beheshti University, Tehran, Iran;Department of Medical Biotechnology, Faculty of Medical Sciences, Tarbiat Modares University, Tehran, Iran;Research and Development Center of Biotechnology, Tarbiat Modares University, Tehran, Iran;

关键词: Single-chain variable fragment (scFv);    camelid VHH;    nanobody;    single-domain antibody;   

DOI  :  10.1186/s40364-021-00332-6

来源: Springer

【 摘 要 】

By the emergence of recombinant DNA technology, many antibody fragments have been developed devoid of undesired properties of natural immunoglobulins. Among them, camelid heavy-chain variable domains (VHHs) and single-chain variable fragments (scFvs) are the most favored ones. While scFv is used widely in various applications, camelid antibodies (VHHs) can serve as an alternative because of their superior chemical and physical properties such as higher solubility, stability, smaller size, and lower production cost. Here, these two counterparts are compared in structure and properties to identify which one is more suitable for each of their various therapeutic, diagnosis, and research applications.

【 授权许可】

CC BY