Evaluation of the effect of vitamin D3 on mandibular condyles in an ovariectomized mouse model: a micro-CT study

BMC Oral Health

Kaan Orhan¹  Csaba Dobó-Nagy²  Szilvia Ambrus³  Szandra Körmendi³  Bálint Vecsei³ 

[1] Department of Dentomaxillofacial Radiology, Faculty of Dentistry, Ankara University, Emniyet Mah.İncitaş sokak.Sabancı Kız yurdu karşısı, 06500, Ankara, Turkey;Department of Oral Diagnostics, Semmelweis University, Szentkirályi u. 47, 1088, Budapest, Hungary;Department of Prosthodontics, Semmelweis University, Szentkirályi u. 47, 1088, Budapest, Hungary;

关键词: Osteoporosis;    Micro-CT;    Condyle;    Vitamin D3;   

DOI  :  10.1186/s12903-021-01980-8

来源: Springer

【 摘 要 】

BackgroundThis study aimed to investigate the effect of ovariectomy and vitamin D3 on bone microstructure; this effect was examined in three regions of interest at one femoral and two mandibular sampling sites bone in an ovariectomized mouse model.MethodsThirty-six week-old female mice were randomly divided into three groups: 10 subjects were given oral cholecalciferol (vitamin D3) daily for 6 weeks after undergoing bilateral ovariectomy (D3 group), while 10 ovariectomized subjects (OVX) and 10 subjects who underwent a sham operation (SHAM) received peanut oil daily during the investigation. After extermination, the left hemimandible and femur were removed and scanned by micro-CT. The bone micromorphology parameters were analyzed and the BMD was calculated.ResultsThe bone volume fraction (BV/TV) was significantly lower in the trabecular bone of the mandibular condyle in the OVX group than in the SHAM and D3 groups. Also there was a significant difference between the SHAM and D3 groups. The specific bone surface (BS/BV) was significantly higher in the OVX and D3 groups than in the SHAM group. Trabecular thickness (Tb.Th) was significantly higher in the SHAM group, and the trabecular bone pattern factor (Tb.Pf) was significantly higher in the OVX group than in the other two groups. Bone mineral density (BMD) of the femur and the mandible was significantly lower in the OVX group than in the SHAM and D3 groups.ConclusionsOur results show that ovariectomy causes a significantly weaker bone microstructure in the mandibular condyle, where the protective effect of vitamin D3 resulted in a partial resorption.

【 授权许可】

CC BY