RNA-seq analysis identified glucose-responsive genes and YqfO as a global regulator in Bacillus subtilis

BMC Research Notes

Mitsuo Ogura¹  Yu Kanesaki² 

[1] Institute of Oceanic Research and Development, Tokai University, 3-20-1 Orido Shimizu-ku, 424-8610, Shizuoka, Japan;Research Institute of Green Science and Technology, Shizuoka University, 836 Ohya, Suruga-ku, 422-8529, Shizuoka, Japan;

关键词: RNA-seq;    Glucose;    Catabolite regulation;    Transcriptome;   

DOI  :  10.1186/s13104-021-05869-1

来源: Springer

【 摘 要 】

ObjectiveWe observed that the addition of glucose enhanced the expression of sigX and sigM, encoding extra-cytoplasmic function sigma factors in Bacillus subtilis. Several regulatory factors were identified for this phenomenon, including YqfO, CshA (RNA helicase), and YlxR (nucleoid-associated protein). Subsequently, the relationships among these regulators were analyzed. Among them, YqfO is conserved in many bacterial genomes and may function as a metal ion insertase or metal chaperone, but has been poorly characterized. Thus, to further characterize YqfO, we performed RNA sequencing (RNA-seq) analysis of YqfO in addition to CshA and YlxR.ResultsWe first performed comparative RNA-seq to detect the glucose-responsive genes. Next, to determine the regulatory effects of YqfO in addition to CshA and YlxR, three pairs of comparative RNA-seq analyses were performed (yqfO/wt, cshA/wt, and ylxR/wt). We observed relatively large regulons (approximately 420, 780, and 180 for YqfO, CshA, and YlxR, respectively) and significant overlaps, indicating close relationships among the three regulators. This study is the first to reveal that YqfO functions as a global regulator in B. subtilis.

【 授权许可】

CC BY