The effect of surface morphology on endothelial and smooth muscle cells growth on blow-spun fibrous scaffolds

Journal of Biological Engineering

Paulina Trzaskowska¹  Aleksandra Kuźmińska²  Iwona Łopianiak²  Michał Wojasiński²  Beata A. Butruk-Raszeja² 

[1] Centre for Advanced Materials and Technologies CEZAMAT, Warsaw University of Technology, Poleczki 19, 02-822, Warsaw, Poland;Laboratory of Biomedical Engineering, Faculty of Chemical and Process Engineering, Warsaw University of Technology, Waryńskiego 1, 00-645, Warsaw, Poland;

关键词: One-layer vascular graft;    Bi-layer vascular graft;    Solution blow spinning;    Polyurethane;    Endothelial cells;    Smooth muscle cells;   

DOI  :  10.1186/s13036-021-00278-1

来源: Springer

【 摘 要 】

This study aimed to analyze the growth of two types of blood vessel building cells: endothelial cells (ECs) and smooth muscle cells (SMCs) on surfaces with different morphology. Two types of materials, differing in morphology, were produced by the solution blow spinning technique. One-layer materials consisted of one fibrous layer with two fibrous surfaces. Bi-layer materials consisted of one fibrous-solid layer and one fibrous layer, resulting in two different surfaces. Additionally, materials with different average fiber diameters (about 200, 500, and 900 nm) were produced for each group. It has been shown that it is possible to obtain structures with a given morphology by changing the selected process parameters (working distance and polymer solution concentration). Both morphology (solid versus fibrous) and average fiber diameter (submicron fibers versus microfibers) of scaffolds influenced the growth of ECs. However, this effect was only visible after an extended period of culture (6 days). In the case of SMCs, it was proved that the best growth of SMCs is obtained for micron fibers (with an average diameter close to 900 nm) compared to the submicron fibers (with an average diameter below 900 nm).

【 授权许可】

CC BY