Mapping pre-harvest sprouting resistance loci in AAC Innova × AAC Tenacious spring wheat population

BMC Genomics

Dean Spaner¹  Jaswinder Singh²  Raman Dhariwal³  Robert J. Graf³  Harpinder S. Randhawa³  Colin W. Hiebert⁴  Mark E. Sorrells⁵ 

[1] Department of Agricultural, Food & Nutritional Science, University of Alberta, T6G 2P5, Edmonton, AB, Canada;Department of Plant Science, McGill University, H9X 3V9, Ste-Anne-de-Bellevue, QC, Canada;Lethbridge Research and Development Centre, Agriculture and Agri-Food Canada, 5403 1st Avenue South, T1J 4B1, Lethbridge, AB, Canada;Morden Research and Development Centre, Agriculture and Agri-Food Canada, 101 Route 100, R6M 1Y5, Morden, MB, Canada;School of Integrative Plant Science, Plant Breeding and Genetics Section, Cornell University, 240 Emerson Hall, 14853, Ithaca, NY, USA;

关键词: Seed dormancy;    Seed coat color;    Phytohormones;    Genetic and epigenetic factors;    Epistasis interactions;   

DOI  :  10.1186/s12864-021-08209-6

来源: Springer

【 摘 要 】

BackgroundPre-harvest sprouting (PHS) is a major problem for wheat production due to its direct detrimental effects on wheat yield, end-use quality and seed viability. Annually, PHS is estimated to cause > 1.0 billion USD in losses worldwide. Therefore, identifying PHS resistance quantitative trait loci (QTLs) is crucial to aid molecular breeding efforts to minimize losses. Thus, a doubled haploid mapping population derived from a cross between white-grained PHS susceptible cv AAC Innova and red-grained resistant cv AAC Tenacious was screened for PHS resistance in four environments and utilized for QTL mapping.ResultsTwenty-one PHS resistance QTLs, including seven major loci (on chromosomes 1A, 2B, 3A, 3B, 3D, and 7D), each explaining ≥10% phenotypic variation for PHS resistance, were identified. In every environment, at least one major QTL was identified. PHS resistance at most of these loci was contributed by AAC Tenacious except at two loci on chromosomes 3D and 7D where it was contributed by AAC Innova. Thirteen of the total twenty-one identified loci were located to chromosome positions where at least one QTL have been previously identified in other wheat genotype(s). The remaining eight QTLs are new which have been identified for the first time in this study. Pedigree analysis traced several known donors of PHS resistance in AAC Tenacious genealogy. Comparative analyses of the genetic intervals of identified QTLs with that of already identified and cloned PHS resistance gene intervals using IWGSC RefSeq v2.0 identified MFT-A1b (in QTL interval QPhs.lrdc-3A.1) and AGO802A (in QTL interval QPhs.lrdc-3A.2) on chromosome 3A, MFT-3B-1 (in QTL interval QPhs.lrdc-3B.1) on chromosome 3B, and AGO802D, HUB1, TaVp1-D1 (in QTL interval QPhs.lrdc-3D.1) and TaMyb10-D1 (in QTL interval QPhs.lrdc-3D.2) on chromosome 3D. These candidate genes are involved in embryo- and seed coat-imposed dormancy as well as in epigenetic control of dormancy.ConclusionsOur results revealed the complex PHS resistance genetics of AAC Tenacious and AAC Innova. AAC Tenacious possesses a great reservoir of important PHS resistance QTLs/genes supposed to be derived from different resources. The tracing of pedigrees of AAC Tenacious and other sources complements the validation of QTL analysis results. Finally, comparing our results with previous PHS studies in wheat, we have confirmed the position of several major PHS resistance QTLs and candidate genes.

【 授权许可】

CC BY