Transcriptional responses of Daphnis nerii larval midgut to oral infection by Daphnis nerii cypovirus-23

Virology Journal

Zhigao Zhan¹  Wendong Kuang¹  Limei Guan¹  Jianghuai Li¹  Jinchang Wang¹  Liang Jin¹  Junhui Chen¹  Xi Zhou²  Chenghua Yan³  Guangqiang Ma³ 

[1] Institute of Microbiology, Jiangxi Academy of Sciences, No. 7777 Changdong Road, 330096, Nanchang, China;Institute of Microbiology, Jiangxi Academy of Sciences, No. 7777 Changdong Road, 330096, Nanchang, China;State Key Laboratory of Virology, Wuhan Institute of Virology, Chinese Academy of Sciences (CAS), 430071, Wuhan, China;School of Life Sciences, Jiangxi University of Traditional Chinese Medicine, 330004, Nanchang, China;

关键词: Daphnis nerii;    Midgut;    Transcriptome analysis;   

DOI  :  10.1186/s12985-021-01721-x

来源: Springer

【 摘 要 】

BackgroundDaphnis nerii cypovirus-23 (DnCPV-23) is a new type of cypovirus and has a lethal effect on the oleander hawk moth, Daphnis nerii which feeds on leave of Oleander and Catharanthus et al. After DnCPV-23 infection, the change of Daphnis nerii responses has not been reported.MethodsTo better understand the pathogenic mechanism of DnCPV-23 infection, 3rd-instar Daphnis nerii larvae were orally infected with DnCPV-23 occlusion bodies and the transcriptional responses of the Daphnis nerii midgut were analyzed 72 h post-infection using RNA-seq.ResultsThe results showed that 1979 differentially expressed Daphnis nerii transcripts in the infected midgut had been identified. KEGG analysis showed that protein digestion and absorption, Toll and Imd signaling pathway were down-regulated. Based on the result, we speculated that food digestion and absorption in insect midgut might be impaired after virus infection. In addition, the down-regulation of the immune response may make D. nerii more susceptible to bacterial infections. Glycerophospholipid metabolism and xenobiotics metabolism were up-regulated. These two types of pathways may affect the viral replication and xenobiotic detoxification of insect, respectively.ConclusionThese results may facilitate a better understanding of the changes in Daphnis nerii metabolism during cypovirus infection and serve as a basis for future research on the molecular mechanism of DnCPV-23 invasion.

【 授权许可】

CC BY