Ionic liquid-based multi-stage sugaring-out extraction of lactic acid from simulated broth and actual lignocellulosic fermentation broth

Bioresources and Bioprocessing

Yi Tong¹  Yi Li¹  Haijun Liu¹  Yang Xu¹  Xiaoyan Wang¹  Xu Zhou²  Yaqin Sun²  Zhilong Xiu²  Hongjun Zhan² 

[1] Jilin COFCO Biochemistry Co., Ltd. (National Engineering Research Center of Corn Deep Processing), 130033, Changchun, Jilin, People’s Republic of China;School of Bioengineering, Dalian University of Technology, No.2 Linggong Road, Ganjingzi District, 116024, Dalian, Liaoning, People’s Republic of China;

关键词: Ionic liquid;    Sugaring-out extraction;    Lactic acid;    Multi-stage;    Fermentation broth;   

DOI  :  10.1186/s40643-021-00481-4

来源: Springer

【 摘 要 】

In this study, ionic liquid-based sugaring-out extraction was developed to separate lactic acid from the synthetic solution and actual lignocellulosic fermentation broth. Except for [EOHmim]BF4, the ILs with BF4− and OTF− anion can form aqueous two-phase system (ATPS) with the aid of saccharides. With the same kind of saccharides, the ATPS formation ability of ILs could be promoted by increasing the side-chain length of ILs in the order of [Hmim]BF4 ≈ [Bmim]BF4 ˃ [Emim]BF4 due to the decrease in ILs’ kosmotropicity. On the other hand, for the same type of ILs, an ATPS was formed more easily with glucose than with xylose. When IL concentration varied from 35% (w/w) to 40% (w/w) at a low glucose concentration of 15% (w/w), an interesting phase reversal was observed. When lactic acid was undissociated at pH 2.0, 51.8% LA and 92.3% [Bmim]BF4 were partitioned to the top phase, and 97.0% glucose to the bottom phase using an ATPS consisting of 25% (w/w) glucose and 45% (w/w) IL. The total recovery of LA would increase to 89.0% in three-stage sugaring-out extraction from synthetic solution. In three-stage sugaring-out extraction from the filtered and unfiltered fermentation broth obtained via simultaneous saccharification and co-fermentation (SSCF) of acid-pretreated corn stover by the microbial consortium, the total recovery of LA was 89.5% and 89.8%, respectively. Furthermore, the total removal ratio of cells and pigments from the unfiltered broth was 68.4% and 65.4%, respectively. The results support IL-based sugaring-out extraction as a potential method for the recovery of lactic acid from actual fermentation broth.Graphical Abstract

【 授权许可】

CC BY