Transcriptome analysis of Rhizopus oryzae seed pellet formation using triethanolamine

Biotechnology for Biofuels

Na Wu¹  Wen Ou¹  Ke Li¹  Qing Xu¹  Ru Wang¹  Yaru Chen¹  Xiao-man Sun¹  He Huang¹  Jiahui Zhang¹  Yingfeng Li¹ 

[1] School of Food Science and Pharmaceutical Engineering, Nanjing Normal University, Nanjing, China;

关键词: R. oryzae;    Seed pellet formation;    Triethanolamine;    Organic acid;    Transcriptomic analysis;   

DOI  :  10.1186/s13068-021-02081-y

来源: Springer

【 摘 要 】

Rhizopus oryzae (R. oryzae) can effectively produce organic acids, and its pellet formation in seed cultures has been shown to significantly enhance subsequent fermentation processes. Despite advances in strain development, simple and effective methods for inducing pellet morphology and a basic understanding of the mechanisms controlling this process could facilitate substantial increases in efficiency and product output. Here, we report that 1.5% triethanolamine (TEOA) in seed culture medium can activate the growth of R. oryzae spores in compact and uniform pellets which is optimal for fermentation conditions. Analysis of fermentation kinetics showed that the production of fumaric and L-malic acid increases 293% and 177%, respectively. Transcriptomic analysis revealed that exposure of R. oryzae to 1.5% TEOA during the seed culture activated the phosphatidylinositol and mitogen-activated protein kinase signaling pathways. Theses pathways subsequently stimulated the downstream carbohydrate-active synthases and hydrolases that required for cell wall component synthesis and reconstruction. Our results thus provide insight into the regulatory pathways controlling pellet morphology germane to the viability of seed cultures, and provide valuable reference data for subsequent optimization of organic acid fermentation by R. oryzae.

【 授权许可】

CC BY