Biotechnology for Biofuels | |
Transcriptome analysis of Rhizopus oryzae seed pellet formation using triethanolamine | |
Na Wu1  Wen Ou1  Ke Li1  Qing Xu1  Ru Wang1  Yaru Chen1  Xiao-man Sun1  He Huang1  Jiahui Zhang1  Yingfeng Li1  | |
[1] School of Food Science and Pharmaceutical Engineering, Nanjing Normal University, Nanjing, China; | |
关键词: R. oryzae; Seed pellet formation; Triethanolamine; Organic acid; Transcriptomic analysis; | |
DOI : 10.1186/s13068-021-02081-y | |
来源: Springer | |
【 摘 要 】
Rhizopus oryzae (R. oryzae) can effectively produce organic acids, and its pellet formation in seed cultures has been shown to significantly enhance subsequent fermentation processes. Despite advances in strain development, simple and effective methods for inducing pellet morphology and a basic understanding of the mechanisms controlling this process could facilitate substantial increases in efficiency and product output. Here, we report that 1.5% triethanolamine (TEOA) in seed culture medium can activate the growth of R. oryzae spores in compact and uniform pellets which is optimal for fermentation conditions. Analysis of fermentation kinetics showed that the production of fumaric and L-malic acid increases 293% and 177%, respectively. Transcriptomic analysis revealed that exposure of R. oryzae to 1.5% TEOA during the seed culture activated the phosphatidylinositol and mitogen-activated protein kinase signaling pathways. Theses pathways subsequently stimulated the downstream carbohydrate-active synthases and hydrolases that required for cell wall component synthesis and reconstruction. Our results thus provide insight into the regulatory pathways controlling pellet morphology germane to the viability of seed cultures, and provide valuable reference data for subsequent optimization of organic acid fermentation by R. oryzae.
【 授权许可】
CC BY
【 预 览 】
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RO202203041548067ZK.pdf | 3278KB | download |