Analysis of nucleotide insertion opposite urea and translesion synthesis across urea by DNA polymerases

Genes and Environment

Takayuki Ohshima¹  Kazuaki Ohara²  Ryuto Anabuki²  Hiroshi Miyazawa²  Takanobu Kobayashi²  Masayuki Morikawa²  Taishu Kawada²  Kyousuke Tokorodani²  Katsuhito Kino² 

[1] Faculty of Science and Engineering, Tokushima Bunri University, 1314-1 Shido, 769-2193, Sanuki, Kagawa, Japan;Kagawa School of Pharmaceutical Sciences, Tokushima Bunri University, 1314-1 Shido, 769-2193, Sanuki, Kagawa, Japan;

关键词: Oxidative DNA damage;    Urea;    DNA polymerase;    Base pair;    Nucleotide incorporation;    Elongation;   

DOI  :  10.1186/s41021-022-00236-3

来源: Springer

【 摘 要 】

Urea (Ua) is produced in DNA as the result of oxidative damage to thymine and guanine. It was previously reported that Klenow fragment (Kf) exo− incorporated dATP opposite Ua, and that DNA polymerase β was blocked by Ua. We report here the following nucleotide incorporations opposite Ua by various DNA polymerases: DNA polymerase α, dATP and dGTP (dATP > dGTP); DNA polymerase δ, dATP; DNA polymerase ζ, dATP; Kf exo−, dATP; Sulfolobus solfataricus P2 DNA polymerase IV (Dpo4), dGTP and dATP (dGTP > dATP); and DNA polymerase η, dCTP, dGTP, dATP, and dTTP (dCTP > dGTP > dATP > dTTP). DNA polymerases β and ε were blocked by Ua. Elongation by DNA polymerases δ and ζ stopped after inserting dATP opposite Ua. Importantly, the elongation efficiency to full-length beyond Ua using DNA polymerase η and Dpo4 were almost the same as that of natural DNA.Graphical abstract

【 授权许可】

CC BY