Complete genome sequence of biocontrol strain Paenibacillus peoriae HJ-2 and further analysis of its biocontrol mechanism

BMC Genomics

Jiangan Hou¹  Chunli Fan¹  Guihe Jiang¹  Jiguang Wei¹  Chengwu Zou¹  Aiming Jiang²  Zunwei Ke³  Jianhua Gong³  Xiang Xu⁴ 

[1] College of Agriculture, Guangxi University, 530004, Nanning, China;College of Agriculture, Guangxi University, 530004, Nanning, China;College of Chemistry and Environmental Engineering, Hanjiang Normal University, 442000, Shiyan, China;College of Chemistry and Environmental Engineering, Hanjiang Normal University, 442000, Shiyan, China;Institute of Basic Medical Sciences, Hubei University of Medicine, 442000, Shiyan, China;

关键词: Paenibacillus peoriae;    Paris polyphylla;    Stem rot;    Genome analysis;    Biocontrol mechanism;   

DOI  :  10.1186/s12864-022-08330-0

来源: Springer

【 摘 要 】

BackgroundParis polyphylla is a herb widely used in traditional Chinese medicine to treat various diseases. Stem rot diseases seriously affected the yield of P. polyphylla in subtropical areas of China. Therefore, cost-effective, chemical-free, eco-friendly strategies to control stem rot on P. polyphylla are valuable and urgently needed.ResultsIn this paper, we reported the biocontrol efficiency of Paenibacillus peoriae HJ-2 and its complete genome sequence. Strain HJ-2 could serve as a potential biocontrol agent against stem rot on P. polyphylla in the greenhouse and field. The genome of HJ-2 consists of a single 6,001,192 bp chromosome with an average GC content of 45% and 5,237 predicted protein coding genes, 39 rRNAs and 108 tRNAs. The phylogenetic tree indicated that HJ-2 is most closely related to P. peoriae IBSD35. Functional analysis of genome revealed numerous genes/gene clusters involved in plant colonization, biofilm formation, plant growth promotion, antibiotic and resistance inducers synthesis. Moreover, metabolic pathways that potentially contribute to biocontrol mechanisms were identified.ConclusionsThis study revealed that P. peoriae HJ-2 could serve as a potential BCA against stem rot on P. polyphylla. Based on genome analysis, the genome of HJ-2 contains more than 70 genes and 12 putative gene clusters related to secondary metabolites, which have previously been described as being involved in chemotaxis motility, biofilm formation, growth promotion, antifungal activity and resistance inducers biosynthesis. Compared with other strains, variation in the genes/gene clusters may lead to different antimicrobial spectra and biocontrol efficacies.

【 授权许可】

CC BY