Single-cell analysis of mouse uterus at the invasion phase of embryo implantation

Cell & Bioscience

Jia-Peng He¹  Ji-Long Liu¹  Qing Tian¹  Qiu-Yang Zhu¹ 

[1] Guangdong Laboratory for Lingnan Modern Agriculture, College of Veterinary Medicine, South China Agricultural University, Tianhe District, No. 483 Wushan Road, 510642, Guangzhou, China;

关键词: Embryo implantation;    Mouse;    Single-cell RNA-seq;    Transcriptional changes;   

DOI  :  10.1186/s13578-022-00749-y

来源: Springer

【 摘 要 】

BackgroundEmbryo implantation into the uterus is a crucial step for human reproduction. A hypothesis has been proposed that the molecular circuit invented by trophoblasts for invasive embryo implantation during evolution might be misused by cancer cells to promote malignancy. Unfortunately, our current understanding of the molecular mechanism underlying embryo implantation is far from complete.ResultsHere we used the mouse as an animal model and generated a single-cell transcriptomic atlas of the embryo implantation site of mouse uterus at the invasion phase of embryo implantation on gestational day 6. We revealed 23 distinct cell clusters, including 5 stromal cell clusters, 2 epithelial cell clusters, 1 smooth muscle cell cluster, 2 pericyte clusters, 4 endothelial cell clusters, and 9 immune cell clusters. Through data analysis, we identified differentially expression changes in all uterine cell types upon embryo implantation. By integrated with single-cell RNA-seq data from E5.5 embryos, we predicted cell–cell crosstalk between trophoblasts and uterine cell types.ConclusionsOur study provides a valuable resource for understanding of the molecular mechanism of embryo implantation.

【 授权许可】

CC BY