Candida albicans Infections: a novel porcine wound model to evaluate treatment efficacy

BMC Microbiology

Michael Solis¹  Stephen C. Davis¹  Joel Gil¹  Alexander Higa¹ 

[1]Miller School of Medicine, Dr. Phillip Frost Department of Dermatology and Cutaneous Surgery Wound Healing Research Laboratory Miami, University of Miami, 33136, Miami, FL, United States

关键词: Candida albicans;    Antifungal;    Porcine;    Wound;    Biofilm model;    New model;    Yeast;    Porcine model;    Infection;    Burns;    Antifungal;   

DOI  :  10.1186/s12866-022-02460-x

来源: Springer

【 摘 要 】

Candida albicans is a common cause of opportunistic mycoses worldwide and a major contributor in wound infections. The purpose of this study was to establish a fungal wound model and analyze the effects of a common antifungal agent against the proliferation of three C. albicans strains. Second degree burns were created, and then inoculated with one of three different C. albicans ATCC strains: 10261 reference strain, 64550 fluconazole resistant and 26310 fluconazole sensitive. After fungal inoculation, every wound was covered with dressings for 4 h to allow fungal colonization on every wound bed. After 4 h, the dressings were removed, and each wound was treated either once or twice daily with a topical terbinafine hydrochloride or left untreated. On days 2, 4 and 7 post inoculation, three wounds from each treatment group were scrub cultured and quantified. On day 2, wounds infected with the sensitive strains 26310 and 10261 and treated twice showed a significant reduction when compared against those infected wounds receiving once daily treatment. On day 4, wounds which were infected with C. albicans fluconazole sensitive (ATCC 26310) showed a significant reduction in fungal cell counts with treatment applied twice daily. A significant reduction in the colony counts was exhibited in all three strains at the seventh day with active as compared to the non-treated wounds. Twice daily treatment resulted in a lower fungal count than once daily treatment. Neither treatment was able to entirely eradicate C. albicans during the duration of this study. Establishing a reliable fungal wound model will help in the translational goal of identifying new antifungal that could be used clinically by wound care providers.

【 授权许可】

CC BY