Single-molecule long-read sequencing reveals the potential impact of posttranscriptional regulation on gene dosage effects on the avian Z chromosome

BMC Genomics

Junpeng Li¹  Liang Li¹  Jianmei Wang¹  Chunchun Han¹  Shengchao Ma¹  Hehe Liu¹  Jiwen Wang¹  Jingjing Qi¹  Rongping Zhang¹  Yang Xi¹ 

[1] Farm Animal Genetic Resources Exploration and Innovation Key Laboratory of Sichuan Province, College of Animal Science and Technology, Sichuan Agricultural University, 613000, Chengdu, China;

关键词: Nanopore;    Posttranscriptional regulation;    Sex chromosomes;    Dose effects;    Duck;   

DOI  :  10.1186/s12864-022-08360-8

来源: Springer

【 摘 要 】

BackgroundMammalian sex chromosomes provide dosage compensation, but avian lack a global mechanism of dose compensation. Herein, we employed nanopore sequencing to investigate the genetic basis of gene expression and gene dosage effects in avian Z chromosomes at the posttranscriptional level.ResultsIn this study, the gonad and head skin of female and male duck samples (n = 4) were collected at 16 weeks of age for Oxford nanopore sequencing. Our results revealed a dosage effect and local regulation of duck Z chromosome gene expression. Additionally, AS and APA achieve tissue-specific gene expression, and male-biased lncRNA regulates its Z-linked target genes, with a positive regulatory role for gene dosage effects on the duck Z chromosome. In addition, GO enrichment and KEGG pathway analysis showed that the dosage effects of Z-linked genes were mainly associated with the cellular response to hormone stimulus, melanin biosynthetic, metabolic pathways, and melanogenesis, resulting in sex differences.ConclusionsOur data suggested that post transcriptional regulation (AS, APA and lncRNA) has a potential impact on the gene expression effects of avian Z chromosomes. Our study provides a new view of gene regulation underlying the dose effects in avian Z chromosomes at the RNA post transcriptional level.

【 授权许可】

CC BY