Advancing the use of molecular methods for routine freshwater macroinvertebrate biomonitoring – the need for calibration experiments

Metabarcoding and Metagenomics

Bastian Egeter¹  Ana Filipa Filipe²  Pedro Beja²  Filipa M. S. Martins³  Vasco Elbrecht⁴  Vera M.A. Zizka⁵  Florian Leese⁵  Pieter Boets⁶  Jan Pawlowski⁷  Markus Majaneva⁸  Elvira Mächler⁹  Rosetta C. Blackman⁹  Florian Altermatt⁹  Cesc Múrria^1,10  Paul L. Schmidt Yáñez^1,11  Kristy Deiner^1,12  Ben Price^1,12  Jan Macher^1,13  Kristian Meissner^1,14  J. Iwan Jones^1,15  Amanda Arnold^1,15 

[1] CIBIO/InBio, Centro de Investigação em Biodiversidade e Recursos Genéticos, Universidade do Porto, Campus de Vairão, Vila do Conde, Portugal;CIBIO/InBio, Centro de Investigação em Biodiversidade e Recursos Genéticos, Universidade do Porto, Campus de Vairão, Vila do Conde, Portugal;CIBIO/InBio, Centro de Investigação em Biodiversidade e Recursos Genéticos, Instituto Superior de Agronomia, Universidade de Lisboa, Lisboa, Portugal;CIBIO/InBio, Centro de Investigação em Biodiversidade e Recursos Genéticos, Universidade do Porto, Campus de Vairão, Vila do Conde, Portugal;Departamento de Biologia, Faculdade de Ciências da Universidade do Porto, Porto, Portugal;Centre for Biodiversity Genomics, University of Guelph, 50 Stone Road E Guelph, ON N1G2W1, Canada;Centre for Water and Environmental Research (ZWU), University of Duisburg-Essen, Universitaetsstraße 3, 45141 Essen, Germany;Aquatic Ecosystem Research Group, University of Duisburg-Essen, Universitaetsstraße 5, 45141 Essen, Germany;Department of Animal Sciences and Aquatic Ecology, Ghent University, Coupure Links 653, B-9000 Ghent, Belgium;Provincial Centre of Environmental Research, Godshuizenlaan 95, 9000 Gent, Belgium;Department of Genetics and Evolution, University of Geneva, Sciences III, 30, Quai, Ernest Ansermet, CH-1211 Geneva, Switzerland;ID-Gene ecodiagnostics, Campus Biotech, 1202 Geneva, Switzerland;Department of Natural History, NTNU University Museum, Norwegian University of Science and Technology, NO-7491, Trondheim, Norway;Eawag, Swiss Federal Institute of Aquatic Science and Technology, Department of Aquatic Ecology, Überlandstrasse 133, CH-8600 Dübendorf, Switzerland;Department of Evolutionary Biology and Environmental Studies, University of Zurich, Winterthurerstr. 190, CH-8057 Zürich, Switzerland;Grup de Recerca Freshwater Ecology, Hydrology and Management (FEHM), Departament de Biologia Evolutiva, Ecologia i Ciències Ambientals, Facultat de Biologia, Universitat de Barcelona, Avinguda Diagonal 643, 08028 Barcelona, Catalonia, Spain;Institut de Recerca de la Biodiversitat (IRBio), Universitat de Barcelona, Barcelona, Catalonia, Spain;Institute of Freshwater Ecology and Inland Fisheries (IGB) 12587 Berlin, Germany;Life Sciences Department, Natural History Museum, Cromwell Road, SW7 5BD, London, UK;Naturalis Biodiversity Center, Marine Biodiversity, Postbus 9517, 2300 RA Leiden;SYKE, Finnish Environment Institute, Programme for Environmental information, Survontie 9 A, FI-40500 Jyväskylä, Finland;School of Biological and Chemical Sciences, Queen Mary University of London, Mile End Road, London E1 4NS, UK;

关键词: Bulk DNA;    community;    DNAqua-Net;    environmental DNA;    experimental methods;    fixative DNA;    monitoring;    Water Framework Directive;   

DOI  :  10.3897/mbmg.3.34735

来源: Pensoft Publishers

【 授权许可】

CC BY