Metabarcoding of soil nematodes: the importance of taxonomic coverage and availability of reference sequences in choosing suitable marker(s)

Metabarcoding and Metagenomics

Melanie Sapp¹  Ian Adams²  Rebecca Lawson²  Thomas Prior²  Matthew Alan Back³  Mohammed Ahmed⁴  Gerrit Karssen⁵ 

[1] Cluster of Excellence on Plant Sciences (CEPLAS), Heinrich Heine University, Population Genetics, 40225 Düsseldorf, Germany., Heinrich Heine University, Düsseldorf, Germany;Fera, Sand Hutton, YO41 1LZ, North Yorkshire, UK., Fera, North Yorkshire, United Kingdom;Harper Adams University, Newport, TF10 8NB, Shropshire, UK., Harper Adams University, Newport, United Kingdom;Harper Adams University, Newport, TF10 8NB, Shropshire, UK., Harper Adams University, Newport, United Kingdom;Swedish Museum of Natural History, Department of Zoology, Stockholm, Sweden., Swedish Museum of Natural History, Stockholm, Sweden;National Plant Protection Organization Geertjesweg 15, 6706 EA, Wageningen, the Netherlands., National Plant Protection Organization, Wageningen, Netherlands;

关键词: barcoding;    taxonomy;    DNA marker;    nuclear;    mitochondrial;    reference database;    primer;   

DOI  :  10.3897/mbmg.3.36408

来源: Pensoft Publishers

【 授权许可】

CC BY