A Volatile and Dynamic Longitudinal Microbiome Is Associated With Less Reduction in Lung Function in Adolescents With Cystic Fibrosis

Frontiers in Cellular and Infection Microbiology

Marisa I. Metzger¹  Sébastien Boutin¹  Simon Y. Graeber²  Marcus A. Mall²  Mirjam Stahl²  Alexander H. Dalpke³  Olaf Sommerburg⁴ 

[1]Department of Infectious Diseases, Medical Microbiology and Hygiene, University of Heidelberg, Heidelberg, Germany

[2]Translational Lung Research Center Heidelberg (TLRC), German Center for Lung Research (DZL), University of Heidelberg, Heidelberg, Germany

[3]German Centre for Lung Research (DZL), Associated Partner Site, Berlin, Germany

[4]Department of Pediatric Respiratory Medicine, Immunology and Critical Care Medicine and Cystic Fibrosis Center, Charité - Universitätsmedizin Berlin, Berlin, Germany

[5]Berlin Institute of Health (BIH), Berlin, Germany

[6]Institute of Medical Microbiology and Virology, Technische Universität Dresden, Dresden, Germany

[7]Translational Lung Research Center Heidelberg (TLRC), German Center for Lung Research (DZL), University of Heidelberg, Heidelberg, Germany

[8]Division of Pediatric Pulmonology & Allergy and Cystic Fibrosis Center, Department of Pediatrics, University of Heidelberg, Heidelberg, Germany

[9]Department of Translational Pulmonology, University of Heidelberg, Heidelberg, Germany

关键词: lung microbiome;    cystic fibrosis;    longitudinal study;    volatility analysis;    Pseudomonas aeruginosa;    adolescent;   

DOI  :  10.3389/fcimb.2021.763121

来源: Frontiers

【 摘 要 】

Progressive impairment in lung function caused by chronic polymicrobial airway infection remains the major cause of death in patients with cystic fibrosis (CF). Cross-sectional studies suggest an association between lung function decline and specific lung microbiome ecotypes. However, longitudinal studies on the stability of the airway microbiome are missing for adolescents with CF constituting the age group showing the highest rate of decline in lung function. In this study, we analyzed longitudinal lung function data and sputum samples collected over a period of 3 to 5 years from 12 adolescents with CF. The sputum microbiome was analyzed using 16S rRNA gene sequencing. Our results indicate that the individual course of the lung microbiome is associated with longitudinal lung function. In our cohort, patients with a dynamic, diverse microbiome showed a slower decline of lung function measured by FEV1% predicted, whereas a more stable and less diverse lung microbiome was related to worse outcomes. Specifically, a higher abundance of the phyla Bacteroidetes and Firmicutes was linked to a better clinical outcome, while Proteobacteria were correlated with a decline in FEV1% predicted. Our study indicates that the stability and diversity of the lung microbiome and the abundance of Bacteroidetes and Firmicutes are associated with the lung function decline and are one of the contributing factors to the disease severity.

【 授权许可】

CC BY