Xbp1 and Brachyury establish an evolutionarily conserved subcircuit of the notochord gene regulatory network

eLife

Diana S José-Edwards¹  Jamie E Kugler¹  Jean-Pierre Saint-Jeannet¹  Karina Braslavskaya¹  Yushi Wu¹  Jermyn Addy¹  Arun Devotta¹  Anna Di Gregorio¹  Lenny J Negrón-Piñeiro¹ 

[1] Department of Molecular Pathobiology, New York University College of Dentistry, New York, United States;

关键词: Ciona robusta;    Xenopus;    notochord;    gene regulatory network;    Brachyury;    XBP1;    C. intestinalis;   

DOI  :  10.7554/eLife.73992

来源: eLife Sciences Publications, Ltd

【 摘 要 】

Gene regulatory networks coordinate the formation of organs and structures that compose the evolving body plans of different organisms. We are using a simple chordate model, the Ciona embryo, to investigate the essential gene regulatory network that orchestrates morphogenesis of the notochord, a structure necessary for the proper development of all chordate embryos. Although numerous transcription factors expressed in the notochord have been identified in different chordates, several of them remain to be positioned within a regulatory framework. Here, we focus on Xbp1, a transcription factor expressed during notochord formation in Ciona and other chordates. Through the identification of Xbp1-downstream notochord genes in Ciona, we found evidence of the early co-option of genes involved in the unfolded protein response to the notochord developmental program. We report the regulatory interplay between Xbp1 and Brachyury, and by extending these results to Xenopus, we show that Brachyury and Xbp1 form a cross-regulatory subcircuit of the notochord gene regulatory network that has been consolidated during chordate evolution.

【 授权许可】

CC BY