Dynamics and nanoscale organization of the postsynaptic endocytic zone at excitatory synapses

eLife

Manon Westra¹  Annemarie ML van Schaik¹  Harold D MacGillavry¹  Lisa AE Catsburg¹ 

[1] Cell Biology, Neurobiology and Biophysics, Department of Biology, Faculty of Science, Utrecht University, Utrecht, Netherlands;

关键词: clathrin;    neuron;    dendrite;    synapse;    super-resolution microscopy;    Rat;   

DOI  :  10.7554/eLife.74387

来源: eLife Sciences Publications, Ltd

【 摘 要 】

At postsynaptic sites of neurons, a prominent clathrin-coated structure, the endocytic zone (EZ), controls the trafficking of glutamate receptors and is essential for synaptic plasticity. Despite its importance, little is known about how this clathrin structure is organized to mediate endocytosis. We used live-cell and super-resolution microscopy to reveal the dynamic organization of this poorly understood clathrin structure in rat hippocampal neurons. We found that a subset of endocytic proteins only transiently appeared at postsynaptic sites. In contrast, other proteins were persistently enriched and partitioned at the edge of the EZ. We found that uncoupling the EZ from the synapse led to the loss of most of these components, while disrupting interactions with the actin cytoskeleton or membrane did not alter EZ positioning. Finally, we found that plasticity-inducing stimuli promoted the reorganization of the EZ. We conclude that the EZ is a stable, highly organized molecular platform where components are differentially recruited and positioned to orchestrate the endocytosis of synaptic receptors.

【 授权许可】

CC BY