Proteome Analysis of the Hypothalamic Arcuate Nucleus in Chronic High-Fat Diet-Induced Obesity

BioMed research international

Chang Yeon Kim¹  Jang Ho Ahn¹  Cherl NamKoong²  Hyung Jin Choi³  Do Hyun Han⁴ 

[1] Functional Neuroanatomy of Metabolism Regulation Laboratory, Department of Anatomy and Cell Biology, Department of Biomedical Sciences, Seoul National University College of Medicine, Seoul, Republic of Korea, snu.ac.kr;Functional Neuroanatomy of Metabolism Regulation Laboratory, Department of Anatomy and Cell Biology, Department of Biomedical Sciences, Seoul National University College of Medicine, Seoul, Republic of Korea, snu.ac.kr;Neuroscience Research Institute, Seoul National University College of Medicine, Seoul, Republic of Korea, snuh.org;Functional Neuroanatomy of Metabolism Regulation Laboratory, Department of Anatomy and Cell Biology, Department of Biomedical Sciences, Seoul National University College of Medicine, Seoul, Republic of Korea, snu.ac.kr;Neuroscience Research Institute, Seoul National University College of Medicine, Seoul, Republic of Korea, snuh.org;Wide River Institute of Immunology, Seoul National University, Hong Cheon, Republic of Korea, snu.ac.kr;Proteomics Core Facility, Biomedical Research Institute, Seoul National University Hospital, Seoul, Republic of Korea, snuh.org;

DOI  :  10.1155/2021/3501770

来源: Hindawi Publishing Corporation

【 摘 要 】

The hypothalamus plays a central role in the integrated regulation of feeding and energy homeostasis. The hypothalamic arcuate nucleus (ARC) contains a population of neurons that express orexigenic and anorexigenic factors and is thought to control feeding behavior via several neuronal circuits. In this study, a comparative proteomic analysis of low-fat control diet- (LFD-) and high-fat diet- (HFD-) induced hypothalamic ARC was performed to identify differentially expressed proteins (DEPs) related to changes in body weight. In the ARC in the hypothalamus, 6621 proteins (FDR<0.01) were detected, and 178 proteins were categorized as DEPs (89 upregulated and 89 downregulated in the HFD group). Among the Gene Ontology molecular function terms associated with the DEPs, protein binding was the most significant. Fibroblast growth factor receptor substrate 2 (Frs2) and SHC adaptor protein 3 (Shc3) were related to protein binding and involved in the neurotrophin signaling pathway according to Kyoto Encyclopedia of Genes and Genomes analysis. Furthermore, high-precision quantitative proteomic analysis revealed that the protein profile of the ARC in mice with HFD-induced obesity differed from that in LFD mice, thereby offering insight into the molecular basis of feeding regulation and suggesting Frs2 and Shc3 as novel treatment targets for central anorexigenic signal induction.

【 授权许可】

Unknown