eLife | |
Daily electrical activity in the master circadian clock of a diurnal mammal | |
Timothy Brown1  Robert J Lucas2  Beatriz Bano-Otalora2  Matthew J Moye3  Casey O Diekman4  Mino DC Belle5  | |
[1] Centre for Biological Timing, Faculty of Biology Medicine and Health, University of Manchester, Manchester, United Kingdom;Division of Diabetes, Endocrinology and Gastroenterology, Faculty of Biology Medicine and Health, University of Manchester, Manchester, United Kingdom;Centre for Biological Timing, Faculty of Biology Medicine and Health, University of Manchester, Manchester, United Kingdom;Division of Neuroscience and Experimental Psychology, Faculty of Biology Medicine and Health, University of Manchester, Manchester, United Kingdom;Department of Mathematical Sciences, New Jersey Institute of Technology, Newark, United States;Department of Quantitative Pharmacology and Pharmacometrics (QP2), Kenilworth, United States;Department of Mathematical Sciences, New Jersey Institute of Technology, Newark, United States;EPSRC Centre for Predictive Modelling in Healthcare, Living Systems Institute, University of Exeter, Exeter, United Kingdom;Institute of Biomedical and Clinical Sciences, University of Exeter Medical School, University of Exeter, Exeter, United Kingdom; | |
关键词: diurnality; circadian rhythms; suprachiasmatic nucleus; electrical activity; mathematical modelling; Other; | |
DOI : 10.7554/eLife.68179 | |
来源: eLife Sciences Publications, Ltd | |
【 摘 要 】
Circadian rhythms in mammals are orchestrated by a central clock within the suprachiasmatic nuclei (SCN). Our understanding of the electrophysiological basis of SCN activity comes overwhelmingly from a small number of nocturnal rodent species, and the extent to which these are retained in day-active animals remains unclear. Here, we recorded the spontaneous and evoked electrical activity of single SCN neurons in the diurnal rodent Rhabdomys pumilio, and developed cutting-edge data assimilation and mathematical modeling approaches to uncover the underlying ionic mechanisms. As in nocturnal rodents, R. pumilio SCN neurons were more excited during daytime hours. By contrast, the evoked activity of R. pumilio neurons included a prominent suppressive response that is not present in the SCN of nocturnal rodents. Our modeling revealed and subsequent experiments confirmed transient subthreshold A-type potassium channels as the primary determinant of this response, and suggest a key role for this ionic mechanism in optimizing SCN function to accommodate R. pumilio’s diurnal niche.
【 授权许可】
CC BY
【 预 览 】
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RO202112116747624ZK.pdf | 6087KB | download |