Local chromatin fiber folding represses transcription and loop extrusion in quiescent cells

eLife

Dakota R Hunt¹  Sarah G Swygert¹  Toshio Tsukiyama¹  Stephanie Portillo-Ledesma²  Tamar Schlick³  Dejun Lin⁴  William S Noble⁵  Cheng-Fu Kao⁶  Po-Yen Lin⁶ 

[1] Basic Sciences Division, Fred Hutchinson Cancer Research Center, Seattle, United States;Department of Chemistry, New York University, New York, United States;Department of Chemistry, New York University, New York, United States;Courant Institute of Mathematical Sciences, New York University, New York, United States;New York University-East China Normal University Center for Computational Chemistry at New York University Shanghai, Shanghai, China;Department of Genome Sciences, University of Washington, Seattle, United States;Department of Genome Sciences, University of Washington, Seattle, United States;Paul G. Allen School of Computer Science and Engineering, University of Washington, Seattle, United States;Institute of Cellular and Organismic Biology, Academia Sinica, Taipei, Taiwan;

关键词: nucleosome;    chromatin;    micro-c;    transcription;    condensin;    quiescence;    S. cerevisiae;   

DOI  :  10.7554/eLife.72062

来源: eLife Sciences Publications, Ltd

【 摘 要 】

A longstanding hypothesis is that chromatin fiber folding mediated by interactions between nearby nucleosomes represses transcription. However, it has been difficult to determine the relationship between local chromatin fiber compaction and transcription in cells. Further, global changes in fiber diameters have not been observed, even between interphase and mitotic chromosomes. We show that an increase in the range of local inter-nucleosomal contacts in quiescent yeast drives the compaction of chromatin fibers genome-wide. Unlike actively dividing cells, inter-nucleosomal interactions in quiescent cells require a basic patch in the histone H4 tail. This quiescence-specific fiber folding globally represses transcription and inhibits chromatin loop extrusion by condensin. These results reveal that global changes in chromatin fiber compaction can occur during cell state transitions, and establish physiological roles for local chromatin fiber folding in regulating transcription and chromatin domain formation.

【 授权许可】

CC BY