Phases of cortical actomyosin dynamics coupled to the neuroblast polarity cycle

eLife

Chet Huan Oon¹  Kenneth E Prehoda¹ 

[1] Institute of Molecular Biology, Department of Chemistry and Biochemistry, University of Oregon, Eugene, United States;

关键词: cell polarity;    actomyosin;    stem cells;    differentiation;    D. melanogaster;   

DOI  :  10.7554/eLife.66574

来源: eLife Sciences Publications, Ltd

【 摘 要 】

The Par complex dynamically polarizes to the apical cortex of asymmetrically dividing Drosophila neuroblasts where it directs fate determinant segregation. Previously, we showed that apically directed cortical movements that polarize the Par complex require F-actin (Oon and Prehoda, 2019). Here, we report the discovery of cortical actomyosin dynamics that begin in interphase when the Par complex is cytoplasmic but ultimately become tightly coupled to cortical Par dynamics. Interphase cortical actomyosin dynamics are unoriented and pulsatile but rapidly become sustained and apically-directed in early mitosis when the Par protein aPKC accumulates on the cortex. Apical actomyosin flows drive the coalescence of aPKC into an apical cap that depolarizes in anaphase when the flow reverses direction. Together with the previously characterized role of anaphase flows in specifying daughter cell size asymmetry, our results indicate that multiple phases of cortical actomyosin dynamics regulate asymmetric cell division.

【 授权许可】

CC BY