The induction of pyrenoid synthesis by hyperoxia and its implications for the natural diversity of photosynthetic responses in Chlamydomonas

eLife

Melinda Frame¹  Alecia Withrow¹  Joseph Weissman²  David M Kramer³  Oliver L Tessmer³  Eric Pollner³  Nicole Norris³  Ben Lucker³  Barbara Sears³  Greg Mogos³  Peter Neofotis³  Jacob Bibik³  David Hall³  Joshua Temple⁴  Sarathi M Weraduwage⁵ 

[1] Center for Advanced Microscopy, Michigan State University, East Lansing, United States;Corporate Strategic Research, ExxonMobil, Annandale, United States;MSU-DOE Plant Research Laboratory, Michigan State University, East Lansing, United States;MSU-DOE Plant Research Laboratory, Michigan State University, East Lansing, United States;Department of Plant Biology, Michigan State University, East Lansing, United States;MSU-DOE Plant Research Laboratory, Michigan State University, East Lansing, United States;Great Lakes Bioenergy Research Center, Michigan State University, East Lansing, United States;

关键词: pyrenoid;    carbon concentrating mechanism;    hyperoxia;    hydrogen peroxide;    photosynthesis;    chloromonas;    Chlamydomonas reinhardtii;   

DOI  :  10.7554/eLife.67565

来源: eLife Sciences Publications, Ltd

【 摘 要 】

In algae, it is well established that the pyrenoid, a component of the carbon-concentrating mechanism (CCM), is essential for efficient photosynthesis at low CO2. However, the signal that triggers the formation of the pyrenoid has remained elusive. Here, we show that, in Chlamydomonas reinhardtii, the pyrenoid is strongly induced by hyperoxia, even at high CO2 or bicarbonate levels. These results suggest that the pyrenoid can be induced by a common product of photosynthesis specific to low CO2 or hyperoxia. Consistent with this view, the photorespiratory by-product, H2O2, induced the pyrenoid, suggesting that it acts as a signal. Finally, we show evidence for linkages between genetic variations in hyperoxia tolerance, H2O2 signaling, and pyrenoid morphologies.

【 授权许可】

CC BY