Influence of macrophage polarization on the effectiveness of surgical therapy of peri-implantitis

International Journal of Implant Dentistry

Frank Schwarz¹  Chantal Khoury¹  Karina Obreja¹  Amira Begic¹  Ausra Ramanauskaite¹  Maria Elisa Galarraga-Vinueza²  Anton Sculean³ 

[1] Department of Oral Surgery and Implantology, Johann Wolfgang Goethe-University, Carolinum, Theodor-Stern-Kai 7, 60596, Frankfurt am Main, Germany;Department of Oral Surgery and Implantology, Johann Wolfgang Goethe-University, Carolinum, Theodor-Stern-Kai 7, 60596, Frankfurt am Main, Germany;Post-Graduate Program in Implant Dentistry (PPGO), Federal University of Santa Catarina (UFSC), Florianópolis, SC, Brazil;Dentistry Faculty, Universidad de Las Américas (UDLA), Quito, Ecuador;Department of Periodontology, School of Dental Medicine, University of Bern, Bern, Switzerland;

关键词: Biopsy;    Dental implant;    Immuno-histochemistry;    Peri-implantitis;    Macrophage polarization;    Combined surgical therapy;   

DOI  :  10.1186/s40729-021-00391-2

来源: Springer

【 摘 要 】

PurposeTo evaluate the influence of macrophage expression and polarization on the effectiveness of surgical therapy of peri-implantitis over a 6 month follow-up.MethodsA total of fourteen patients (n = 14 implants) diagnosed with peri-implantitis underwent access flap surgery, granulation tissue removal, implantoplasty, and augmentation at intra-bony components using a natural derived bone mineral and application of a native collagen membrane during a standardized surgical procedure. Granulation tissue biopsies were prepared for immunohistochemical characterization and macrophage polarization assessment. M1 and M2 phenotype expression was identified and quantified through immunohistochemical markers and histomorphometrical analyses. Clinical evaluation and data collection were performed initially and after a healing period of 6 months. Statistical analyses were performed to associate infiltrated area, macrophage, and M1/M2 phenotype influence on peri-implant tissue healing parameters after a 6-month follow-up.ResultsMean infiltrated compartment (ICT) values occupied a total percentage of 70.3% ± 13.0 in the analyzed granulation tissue biopsies. Macrophages occupied a mean area of 15.3% ± 7.0. M1 and M2 phenotypes were present in 7.1 ± 4.1% and 5.5 ± 3.7%, respectively. No statistically significant difference was observed between M1 and M2% expression (p = 0.16). The mean M1/ M2 ratio amounted to 1.5 ± 0.8. Surgical therapy was associated with statistically significant reductions in mean bleeding on probing (BOP), probing depth (PD) and suppuration (SUPP) scores at 6 months (p < 0.05). Linear regression analyses revealed a significant correlation between macrophage expression (CD68%) and changes in PD scores and M1 (%) expression and changes in mucosal recession (MR) scores at 6 months.ConclusionsThe present data suggest that macrophages might influence peri-implant tissue healing mechanisms following surgical therapy of peri-implantitis over a short-term period. Particularly, changes in PD and MR scores were statistically significantly associated with macrophage expression and phenotype.Graphical Abstract

【 授权许可】

CC BY