Persistence of SARS-CoV-2 infection on personal protective equipment (PPE)

BMC Infectious Diseases

Francisco Rodríguez-Esparragón¹  Bernardino Clavo²  Omar García-Pérez³  Sara Cazorla-Rivero³  Elizabeth Córdoba-Lanús⁴  José-Enrique Piñero⁵  Jacob Lorenzo-Morales⁵ 

[1] CIBER de Enfermedades Infecciosas, Instituto de Salud Carlos III, 28029, Madrid, Spain;Research Unit, Hospital Universitario Dr. Negrín, Las Palmas de Gran Canaria, Spain;Fundación Canaria del Instituto de Investigación Sanitaria de Canarias (FIISC), Las Palmas de Gran Canaria, Spain;CIBER de Enfermedades Infecciosas, Instituto de Salud Carlos III, 28029, Madrid, Spain;Research Unit, Hospital Universitario Dr. Negrín, Las Palmas de Gran Canaria, Spain;Fundación Canaria del Instituto de Investigación Sanitaria de Canarias (FIISC), Las Palmas de Gran Canaria, Spain;Chronic Pain Unit, Hospital Universitario Dr. Negrín, Las Palmas de Gran Canaria, Spain;Radiation Oncology Department, Hospital Universitario Dr. Negrín, Las Palmas de Gran Canaria, Spain;RETIC de Investigación en Servicios de Salud en Enfermedades Crónicas (REDISSEC), Instituto de Salud Carlos III, Madrid, Spain;Instituto Universitario de Investigaciones Biomédicas y Sanitarias (IUIBS), BioPharm Group, Universidad de Las Palmas de Gran Canaria, Las Palmas de Gran Canaria, Spain;Instituto Universitario de Enfermedades Tropicales y Salud Pública de Canarias de La Universidad de La Laguna, La Laguna, Tenerife, Spain;Departamento de Medicina Interna, Dermatología y Psiquiatría, Universidad de La Laguna, La Laguna, Tenerife, Spain;Red Cooperativa de Enfermedades Tropicales (RICET), Instituto de Salud Carlos III, Madrid, Spain;Instituto Universitario de Enfermedades Tropicales y Salud Pública de Canarias de La Universidad de La Laguna, La Laguna, Tenerife, Spain;Departamento de Medicina Interna, Dermatología y Psiquiatría, Universidad de La Laguna, La Laguna, Tenerife, Spain;Red Cooperativa de Enfermedades Tropicales (RICET), Instituto de Salud Carlos III, Madrid, Spain;CIBER de Enfermedades Infecciosas, Instituto de Salud Carlos III, 28029, Madrid, Spain;Instituto Universitario de Enfermedades Tropicales y Salud Pública de Canarias de La Universidad de La Laguna, La Laguna, Tenerife, Spain;Red Cooperativa de Enfermedades Tropicales (RICET), Instituto de Salud Carlos III, Madrid, Spain;CIBER de Enfermedades Infecciosas, Instituto de Salud Carlos III, 28029, Madrid, Spain;Departamento de Obstetricia, Ginecología, Pediatría, Medicina Preventiva y Salud Pública, Toxicología, Medicina Legal y Forense y Parasitología, Universidad de La Laguna, La Laguna, Tenerife, Spain;

关键词: COVID-19;    SARS-CoV-2;    RNA;    PPE;    Personal protective equipment;    Face masks;   

DOI  :  10.1186/s12879-021-06861-7

来源: Springer

【 摘 要 】

BackgroundSARS-CoV-2 stability and infection persistence has been studied on different surfaces, but scarce data exist related to personal protective equipment (PPE), moreover using realist viral loads for infection. Due to the importance for adequate PPE management to avoid risk of virus infection, RNA stability was evaluated on PPE.MethodsPersistence of SARS-CoV-2 infection and detection of genomic RNA in PPE (gowns and face masks) were determined by in-vitro assays and RT-qPCR, respectively. Samples were infected with a clinical sample positive for SARS-CoV-2 (Clin-Inf), and with a heat-inactivated SARS-CoV-2 strain sample (Str-Inf) as a control.ResultsPPE samples infected with Clin-Inf were positive for the 3 viral genes on gowns up to 5 days post-infection, whereas these overall genes were detected up to 30 days in the case of face masks. However, gowns and FFP2 masks samples contaminated with Clin-Inf showed a cytopathic effect over VERO cells up to 5–7 days post-infection.ConclusionsSARS-CoV-2 RNA was detected on different PPE materials for 5 to 30 days, but PPE contaminated with the virus was infectious up to 5–7 days. These findings demonstrate the need to improve PPE management and to formulate strategies to introduce viricidal compounds in PPE fabrics.

【 授权许可】

CC BY