Cargo proteins in extracellular vesicles: potential for novel therapeutics in non-alcoholic steatohepatitis

Journal of Nanobiotechnology

Seon-Yeong Jeong¹  Seul Ki Lee¹  Soo Kim¹  Jimin Kim¹  Hye Jin Cho²  Joonghoon Park³  Tae Min Kim³ 

[1] Brexogen Research Center, Brexogen Inc., Songpa-gu, 05855, Seoul, South Korea;Graduate School of International Agricultural Technology, Seoul National University, 25354, Pyeongchang, Gangwon-do, South Korea;Graduate School of International Agricultural Technology, Seoul National University, 25354, Pyeongchang, Gangwon-do, South Korea;Institutes of Green-Bio Science and Technology, Seoul National University, 25354, Pyeongchang, Gangwon-do, South Korea;

关键词: Non-alcoholic steatohepatitis;    Induced mesenchymal stem cells;    Extracellular vesicles;    Steatosis;    Inflammation;    Regeneration;   

DOI  :  10.1186/s12951-021-01120-y

来源: Springer

【 摘 要 】

BackgroundExtracellular vesicles (EVs) are recognized as novel cell-free therapeutics. Non-alcoholic steatohepatitis (NASH) remains a critical health problem. Herein, we show that EVs from pan peroxisome proliferator-activated receptor agonist-primed induced mesenchymal stem cell (pan PPAR-iMSC-EVs) has unique cargo protein signatures, and demonstrate its therapeutic function in NASH.ResultsA unique protein signatures were identified in pan PPAR-iMSC-EVs against those from non-stimulated iMSC-EVs. NASH mice receiving pan PPAR-iMSC-EVs showed reduced steatotic changes and ameliorated ER stress and mitochondiral oxidative stress induced by inflammation. Moreover, pan PPAR-iMSC-EVs promoted liver regeneration via inhibiting apoptosis and enhancing proliferation.ConclusionsWe conclude that our strategy for enriching unique cargo proteins in EVs may facilitate the development of novel therapeutic option for NASH.Graphical Abstract

【 授权许可】

CC BY