Transcriptome sequencing identified the ceRNA network associated with recurrent spontaneous abortion

BMC Medical Genomics

Yuan Liao¹  Ying Hu¹  Yong Huang¹  Juan Li¹  Hui Zou¹  Kaiju Wang¹  Jiayuan Hao¹  Lihua Zhou¹ 

[1]Department of Reproductive Medicine, The Second Affiliated Hospital of Hainan Medical University, No. 368, Yehai Avenue, 570311, Haikou, Hainan, People’s Republic of China

关键词: Recurrent spontaneous abortion;    Transcriptome sequencing;    lncRNA;    ceRNA network;   

DOI  :  10.1186/s12920-021-01125-4

来源: Springer

【 摘 要 】

BackgroundRecurrent spontaneous abortion (RSA) is one of the common complication of pregnancy, bringing heavy burden to the patients and their families. The study aimed to explore the lncRNA-miRNA-mRNA network associated with recurrent spontaneous abortion.MethodsBy transcriptome sequencing, we detected differences in lncRNA, miRNA and mRNA expression in villus tissue samples collected from 3 patients with RSA and 3 normal abortion patients. Differentially expressed lncRNAs, miRNAs and genes (DELs, DEMs and DEGs, respectively) were identified, and Geno Ontology (GO) and Kyoto Encyclopedia of Genes and Genomes (KEGG) analyses were used to determine the functions of DELs and DEGs, which were analysed by Fisher’s test. We also observed the regulatory relationships between miRNA-mRNA and lncRNA-miRNA by Cytoscape 3.6.1.ResultsThe results showed that 1008 DELs (523 upregulated and 485 downregulated), 475 DEGs (201 upregulated and 274 downregulated) and 37 DEMs (15 upregulated and 22 downregulated) were identified. And we also constructed a novel lncRNA-related ceRNA network containing 31 lncRNAs, 1 miRNA (hsa-miR-210-5p) and 3 genes (NTNG2, GRIA1 and AQP1).ConclusionslncRNA-related ceRNA network containing 31 lncRNAs, 1 miRNA (hsa-miR-210-5p) and 3 mRNAs (NTNG2, GRIA1 and AQP1) was constructed. The results may provide a basic theory for elucidating the mechanism underlying RSA.

【 授权许可】

CC BY