Metagenomic analysis to identify novel infectious agents in systemic anaplastic large cell lymphoma

Infectious Agents and Cancer

Mia Steinberg¹  Joseph Boland¹  Charles F. Lynch²  Jason Nomburg³  Joo Y. Song⁴  Dennis D. Weisenburger⁴  Susan Bullman⁵  Eric A. Engels⁶  Parag Mahale⁶  Gabriel Starrett⁷  Amy Chadburn⁸  Paul G. Rubinstein⁹  Brenda Y. Hernandez^1,10 

[1] Cancer Genomics Research Laboratory, National Cancer Institute, Rockville, MD, USA;Department of Epidemiology, The University of Iowa College of Public Health, Iowa City, Iowa, USA;Department of Medical Oncology, Dana-Farber Cancer Institute, Harvard Medical School, Boston, MA, USA;Department of Pathology, City of Hope National Medical Center, Duarte, CA, USA;Human Biology Division, Fred Hutchinson Cancer Research Center, Seattle, WA, USA;Infections and Immunoepidemiology Branch, Division of Cancer Epidemiology and Genetics, National Cancer Institute, Rockville, MD, USA;Laboratory of Cellular Oncology, Center for Cancer Research, National Cancer Institute, Bethesda, MD, USA;Pathology and Laboratory Medicine, Weill Cornell Medical College, New York, NY, USA;Stroger Hospital of Cook County, Ruth M. Rothstein Core Center, Rush University Medical Center, Chicago, IL, USA;University of Hawaii Cancer Center, Honolulu, HI, USA;

关键词: Lymphoma;    Viruses;    Metagenomics;    Immunosuppression;   

DOI  :  10.1186/s13027-021-00404-0

来源: Springer

【 摘 要 】

Systemic anaplastic large cell lymphoma (ALCL) is a rare CD30-expressing T-cell non-Hodgkin lymphoma. Risk of systemic ALCL is highly increased among immunosuppressed individuals. Because risk of cancers associated with viruses is increased with immunosuppression, we conducted a metagenomic analysis of systemic ALCL to determine whether a known or novel pathogen is associated with this malignancy. Total RNA was extracted and sequenced from formalin-fixed paraffin-embedded tumor specimens from 19 systemic ALCL cases (including one case from an immunosuppressed individual with human immunodeficiency virus infection), 3 Epstein-Barr virus positive diffuse large B-cell lymphomas (DLBCLs) occurring in solid organ transplant recipients (positive controls), and 3 breast cancers (negative controls). We used a pipeline based on the Genome Analysis Toolkit (GATK)-PathSeq algorithm to subtract out human RNA reads and map the remaining RNA reads to microbes. No microbial association with ALCL was identified, but we found Epstein-Barr virus in the DLBCL positive controls and determined the breast cancers to be negative. In conclusion, we did not find a pathogen associated with systemic ALCL, but because we analyzed only one ALCL tumor from an immunosuppressed person, we cannot exclude the possibility that a pathogen is associated with some cases that arise in the setting of immunosuppression.

【 授权许可】

CC BY