A short primer on lung stereology

Respiratory Research

Matthias Ochs¹  Julia Schipke² 

[1] Institute of Functional Anatomy, Charité-Universitätsmedizin Berlin, Corporate Member of Freie Universität Berlin and Humboldt Universität zu Berlin, Philippstr. 11, 10115, Berlin, Germany;German Center for Lung Research (DZL), Berlin, Germany;Institute of Functional and Applied Anatomy, Hannover Medical School, Hannover, Germany;Biomedical Research in Endstage and Obstructive Lung Disease, Member of the German Center for Lung Research (DZL), Hannover, Germany;

关键词: Alveoli;    Dimension trap;    Disector;    Histology;    Lung;    Microscopy;    Morphometry;    Reference trap;    Sampling;    Stereology;   

DOI  :  10.1186/s12931-021-01899-2

来源: Springer

【 摘 要 】

The intention of this short primer is to raise your appetite for proper quantitative assessment of lung micro-structure. The method of choice for obtaining such data is stereology. Rooted in stochastic geometry, stereology provides simple and efficient tools to obtain quantitative three-dimensional information based on measurements on nearly two-dimensional microscopic sections. In this primer, the basic concepts of stereology and its application to the lung are introduced step by step along the workflow of a stereological study. The integration of stereology in your laboratory work will help to improve its quality. In a broader context, stereology may also be seen as a contribution to good scientific practice.

【 授权许可】

CC BY