Three-channel ion chromatograph for improved metabolic evaluation of urolithiasis

BMC Urology

Wenbo Tang¹  Guanlin Liu²  Yue Cheng²  Qiang Li² 

[1] Department of Gynecology, Ningbo Women and Children’s Hospital, Zhejiang University, Ningbo, Zhejiang, People’s Republic of China;Department of Urology, Ningbo First Hospital, Zhejiang University, Ningbo, Zhejiang, People’s Republic of China;

关键词: Citrate;    Ion chromatograph;    Metabolic evaluation;    Oxalate;    Urinary calculus;    Urolithiasis;   

DOI  :  10.1186/s12894-021-00914-4

来源: Springer

【 摘 要 】

BackgroundUrolithiasis is a multi-etiological disease resulting from a combination of environmental and genetic factors. One of the most challenging aspects of this disease is its high recurrence rate. For most patients, an in-depth metabolic evaluation may reveal the presence of urinary stones. The fact that different urinary stone-related compounds (USRCs) are measured by different methods renders the metabolic evaluation of urolithiasis quite tedious and complex.MethodsA three-channel ion chromatograph (IC) that automatically measures the concentration of common metabolic indicators of urolithiasis in urine (i.e., oxalate, citrate, uric acid, calcium, and magnesium) was developed to improve the efficiency. To validate its precision and specificity, standard curves were prepared using working solution of these indicators. 100 standard solutions of these indicators were measured with our new IC and three other ICs as the control instruments; analyte concentrations in 100 24-h urine samples from volunteers and 135 calculi patients were also measured.ResultsAll analytes had good linear relationships in concentration ranges of 0–10 mg/L. The precision experiments in the standard and urine samples showed that the measurement errors of the newly developed IC were all less than 5%. In urine, the recovery rate ranged from 99.6 to 100.4%, the coefficient of variation ranged from 1.39 to 2.99%, and the results matched between our newly developed IC and the control ICs. The results of the efficiency test showed that we can finish the analysis at the average number of 14 people per day with the new IC. While the average number in the control group is 3.85/day (p = 0.000).ConclusionsOverall, this multi-channel system significantly improves the efficiency of metabolic evaluation while retaining accuracy and precision.

【 授权许可】

CC BY