Effectiveness of a novel orally administered combination drug product containing milbemycin oxime and lotilaner (Credelio® Plus) for the treatment of larval and immature adult stages of Ancylostoma caninum in experimentally infected dogs

Parasites & Vectors

Dwight D. Bowman¹  Daniel E. Snyder²  Craig R. Reinemeyer³  Elizabeth Crawley⁴  Kim Wallace⁴  Scott Wiseman⁵ 

[1]College of Veterinary Medicine, Cornell University, Ithaca, NY, USA

[2]Daniel E. Snyder DVM PhD. Consulting, LLC, 46229, Indianapolis, IN, USA

[3]East Tennessee Clinical Research Inc., Rockwood, TN, USA

[4]Elanco Animal Health Research and Development, 2500 Innovation Way, 46140, Greenfield, IN, USA

[5]Elanco Animal Health, Priestley Road Basingstoke, RG24 9NL, Hants, UK

关键词: Ancylostoma caninum;    Chemoprophylaxis;    Credelio Plus;    Dog;    Effectiveness;    Hookworm;    Immature;    Larval;    Lotilaner;    Milbemycin oxime;    Oral;    Nematodes;   

DOI  :  10.1186/s13071-021-04761-y

来源: Springer

【 摘 要 】

BackgroundThe hookworm, Ancylostoma caninum, is a common and important zoonotic intestinal nematode parasite that infects dogs globally. Both the immature and adult stages of A. caninum ingest large volumes of blood during the feeding process and can cause severe anemia and death in young dogs, even before patent infections can be diagnosed using routine faecal examination methods. Thus, effective treatment of any pre-patent stages of immature hookworms can reduce or eliminate the risk of clinical disease in infected dogs and additionally reduce environmental contamination of eggs and infective larvae. Two randomized, blinded, GCP-compliant, pivotal laboratory dose confirmation studies were conducted to evaluate the effectiveness and safety of a new novel combination of lotilaner and milbemycin oxime tablets (Credelio Plus®) administered orally to dogs experimentally infected with immature (L4 and immature adult [L5]) stages of A. caninum.MethodsTreatments using the intended global commercial tablet formulation of Credelio Plus were administered in a time frame relative to inoculation with infective larvae so that effectiveness could be assessed against each specific immature stage of A. caninum. In each study, dogs were randomized to one of six (study 1) or four (study 2) treatment groups. Each treatment group contained 8 (study 1) or 10 (study 2) dogs that had been experimentally inoculated with infective A. caninum larvae on day 0 and were dosed once on day 7 or day 11. Enrolled subjects were administered placebo tablets, Credelio Plus tablets, or lotilaner mono tablets to provide minimum dosages of 0.75 mg/kg of milbemycin oxime and 20 mg/kg of lotilaner. All dogs were necropsied 5 days after their respective treatment. All nematodes recovered from the gastrointestinal tract at necropsy were counted by species and stage.ResultsFor both dose confirmation studies and based on geometric mean worm counts, efficacy of Credelio Plus was ≥ 97.3% against L4 larval stage of A. caninum and ≥ 98.7% against immature adult (L5) A. caninum.ConclusionsThese studies demonstrated that the orally administered Credelio Plus combination tablet was highly efficacious in treating immature (L4 and immature adult [L5]) stages of A. caninum in experimentally infected dogs.Graphical Abstract

【 授权许可】

CC BY