Comparison of sampling methods in assessing the microbiome from patients with ulcerative colitis

BMC Gastroenterology

In-Cheol Yoon¹  Sam-Ryong Jee²  Dan Kim²  Hong Sub Lee²  Sang-Heon Lee²  Jun-Sik Yoon²  Sung Jae Park²  Jun-Young Jung²  Seung Jung Yu²  Hyun-Seok Oh³ 

[1] Department of Gastroenterology, Myongji Hospital, Hanyang University College of Medicine, Goyang, Korea;Department of Internal Medicine, Inje University College of Medicine, Busan Paik Hospital, 75 Bokji-ro, Busanjin-gu, 47392, Busan, Korea;Interdisciplinary Program in Bioinformatics, Seoul National University, 08826, Seoul, Korea;ChunLab Inc, 06725, Seoul, Korea;

关键词: Colon lavage;    Colonoscopy;    Inflammatory bowel disease;    Microbiome;    Ulcerative colitis;   

DOI  :  10.1186/s12876-021-01975-3

来源: Springer

【 摘 要 】

BackgroundDysbiosis of ulcerative colitis (UC) has been frequently investigated using readily accessible stool samples. However, stool samples might insufficiently represent the mucosa-associated microbiome status. We hypothesized that luminal contents including loosely adherent luminal bacteria after bowel preparation may be suitable for diagnosing the dysbiosis of UC.MethodsThis study included 16 patients with UC (9 men and 7 women, mean age: 52.13 ± 14.09 years) and 15 sex- and age-matched healthy individuals (8 men and 7 women, mean age: 50.93 ± 14.11 years). They donated stool samples before colonoscopy and underwent luminal content aspiration and endoscopic biopsy during the colonoscopy. Then, the composition of each microbiome sample was analyzed by 16S rRNA-based next-generation sequencing.ResultsThe microbiome between stool, luminal contents, and biopsy was significantly different in alpha and beta diversities. However, a correlation existed between stool and luminal contents in the Procrustes test (p = 0.001) and Mantel test (p = 0.0001). The stool microbiome was different between patients with UC and the healthy controls. Conversely, no difference was found in the microbiome of luminal content and biopsy samples between the two subject groups. The microbiome of stool and lavage predicted UC, with AUC values of 0.85 and 0.81, respectively.ConclusionThe microbiome of stool, luminal contents, and biopsy was significantly different. However, the microbiome of luminal contents during colonoscopy can predict UC, with AUC values of 0.81. Colonoscopic luminal content aspiration analysis could determine microbiome differences between patients with UC and the healthy control, thereby beneficial in screening dysbiosis via endoscopy.Trial registration: This trial was registered at http://cris.nih.go.kr. Registration No.: KCT0003352), Date: 2018–11-13.

【 授权许可】

CC BY