Effect of interface-active proteins on the salt crystal size in waterborne hybrid materials

Applied Adhesion Science

Karsten Thiel¹  Michael Noeske¹  Welchy Leite Cavalcanti¹  Vinicius Carrillo Beber¹  Peter Schiffels¹  Stefan Dieckhoff¹  Kwasi Boateng²  Stephani Stamboroski³  Ingo Grunwald⁴  Dorothea Brüggemann⁵ 

[1] Fraunhofer Institute for Manufacturing Technology and Advanced Materials IFAM, Wiener Straße 12, 28359, Bremen, Germany;Fraunhofer Institute for Manufacturing Technology and Advanced Materials IFAM, Wiener Straße 12, 28359, Bremen, Germany;Institute for Biophysics, University of Bremen, Otto-Hahn-Allee 1, 28359, Bremen, Germany;Fraunhofer Institute for Manufacturing Technology and Advanced Materials IFAM, Wiener Straße 12, 28359, Bremen, Germany;Institute for Biophysics, University of Bremen, Otto-Hahn-Allee 1, 28359, Bremen, Germany;Department Adhesion and Interface Research, Fraunhofer Institute for Manufacturing Technology and Advanced Materials, Wiener Straße 12, 28359, Bremen, Germany;Hochschule Bremen, City University of Applied Sciences, Neustadtswall 30, 28199, Bremen, Germany;Purenum GmbH, Fahrenheitstraße 1, 28359, Bremen, Germany;Institute for Biophysics, University of Bremen, Otto-Hahn-Allee 1, 28359, Bremen, Germany;MAPEX Center for Materials and Processes, University of Bremen, 28359, Bremen, Germany;

关键词: Protein-based composites;    Sodium chloride;    Bovine serum albumin;    Collagen;    Fibrinogen;    Crystallization;    Spatiotemporal development of adhesion;    Damköhler number;   

DOI  :  10.1186/s40563-021-00137-8

来源: Springer

【 摘 要 】

Aqueous processes yielding hybrid or composite materials are widespread in natural environments and their control is fundamental for a multiplicity of living organisms. Their design and in vitro engineering require knowledge about the spatiotemporal evolution of the interactions between the involved liquid and solid phases and, especially, the interphases governing the development of adhesion during solidification. The present study illustrates the effects of distinct proteins on the precipitation of sodium chloride encompassing the size, shape and distribution of halite crystals formed during the drying of droplets containing equally concentrated saline protein solutions. The precipitates obtained from aqueous sodium chloride formulations buffered with tris(hydroxymethyl)aminomethane (Tris) contained either bovine serum albumin (BSA), fibrinogen or collagen and were characterized with respect to their structure and composition using optical and electron microscopy as well as x-ray analysis. The acquired findings highlight that depending on the protein type present during droplet drying the halite deposits predominantly exhibit cubic or polycrystalline dendritic structures. Based on the phenomenological findings, it is suggested that the formation of the interphase between the growing salt phase and the highly viscous saline aqueous jelly phase containing protein governs not only the material transport in the liquid but also the material exchange between the solid and liquid phases.

【 授权许可】

CC BY