Low frequency of HPV positivity in breast tumors among patients from south-central Poland

Infectious Agents and Cancer

Dorota Słonina¹  Beata Biesaga²  Aleksandra Grela-Wojewoda³  Marek Ziobro³  Marta Kołodziej-Rzepa⁴  Anna Mucha-Małecka⁵  Anna Janecka-Widła⁶  Joanna Wysocka⁶  Kaja Majchrzyk⁶  Aleksandra Ambicka⁶  Agnieszka Harazin-Lechowska⁶  Agnieszka Adamczyk⁶  Joanna Niemiec⁷ 

[1] Center for Translational Research and Molecular Biology of Cancer, Maria Sklodowska-Curie National Research Institute of Oncology, Gliwice Branch, Wybrzeże Armii Krajowej 15, 44-101, Gliwice, Poland;Center for Translational Research and Molecular Biology of Cancer, Maria Sklodowska-Curie National Research Institute of Oncology, Gliwice Branch, Wybrzeże Armii Krajowej 15, 44-101, Gliwice, Poland;Department of Tumor Pathology, Maria Sklodowska-Curie National Research Institute of Oncology, Cracow Branch, Gliwice, Poland;Department of Clinical Oncology, Maria Sklodowska-Curie National Research Institute of Oncology, Cracow Branch, Gliwice, Poland;Department of General, Oncological and Vascular Surgery, 5th Military Clinical Hospital, Cracow, Poland;Department of Radiotherapy, Maria Sklodowska-Curie National Research Institute of Oncology, Cracow Branch, Gliwice, Poland;Department of Tumor Pathology, Maria Sklodowska-Curie National Research Institute of Oncology, Cracow Branch, Gliwice, Poland;Institute of Medical Sciences, Medical College of Rzeszow University, Rzeszow, Poland;Department Laboratory of Medical Diagnostics, Cytogenetics and Flow Cytometry Specialist Hospital, Brzozow, Poland;

关键词: Breast cancers;    HPV infection;    South-central Poland;   

DOI  :  10.1186/s13027-021-00405-z

来源: Springer

【 摘 要 】

BackgroundSome studies suggest that Human Papilloma Virus (HPV) infection is important factor in carcinogenesis of breast tumors. This study’ objective was to analyze HPV prevalence in breast cancers of patients from south-central Poland.Materials and methodsThe study was performed based on archival paraffin embebbed and formalin fixed blocks in the group of 383 patients with breast cancer. HPV prevalence and its genotype were assessed, respectively by: nested PCR (with two groups of primers: PGMY09/PGMY11 and GP5+/GP6+), quantitative PCR (qPCR). Tumors were classified as HPV positive in case of at least one positive result in nested PCR and positive results in genotyping procedure. For all HPV positive tissues P16 immunostaining was applied in order to confirm active viral infection.ResultsIn the group of 383 breast cancers, HPV positivity was found in 17 samples (4.4%) in nested PCR. All these samples were subjected to HPV genotyping. This analysis revealed presence of HPV type 16 into two tumors (0.5%). In these two cancers, P16 overexpression was reported.ConclusionIn breast tumors of patients from south-central Poland in Poland, HPV positivity is demonstrated in very low percentage of cases.

【 授权许可】

CC BY