LncRNA DLEU1 is overexpressed in premature ovarian failure and sponges miR-146b-5p to increase granulosa cell apoptosis

Journal of Ovarian Research

Zhihong Qin¹  Xiaoqian Zhang²  Caihong Zheng³  Shiwei Liu³  Yubao Song⁴ 

[1] Department of Endocrinology, Huhhot First Hospital, 010020, Huhhot, The Inner Mongolia, Autonomous Region, People’s Republic of China;Department of Endocrinology, Jincheng General Hospital, 048006, Jincheng City, Shanxi Province, People’s Republic of China;Department of Endocrinology, Shanxi Bethune Hospital, 030032, Taiyuan City, Shanxi Province, People’s Republic of China;Second Department of General Surgery, Shanxi Provincial Cancer Hospital, No. 3, Xincun Worker’s Village, Xinghualing District, 030013, Taiyuan City, Shanxi Province, People’s Republic of China;

关键词: DLEU1;    POF;    miR-146b-5p;    Premature ovarian failure;   

DOI  :  10.1186/s13048-021-00905-x

来源: Springer

【 摘 要 】

BackgroundmiR-146b-5p has been reported to participate in premature ovarian failure (POF) in mice. However, its role in POF patients is unclear. We predicted that miR-146b-5p might interact with lncRNA DLEU1, a crucial player in ovarian cancer. We then explored the interaction between DLEU1 and miR-146b-5p.MethodsExpression of DLEU1 and miR-146b-5p in POF and control ovary tissues was determined by RT-qPCR. The subcellular location of DLEU1 in human KGN cells was analyzed using subcellular fractionation assays. The direct interaction between DLEU1 and miR-146b-5p was analyzed using RNA pull-down assays. The role of DLEU1 in miR-146a expression was analyzed using overexpression assay. Cell proliferation was analyzed using cell apoptosis assay.ResultsIncreased DLEU1 expression and decreased miR-146b-5p expression were observed in POF. DLEU1 directly interacted with MiR-146b-5p and was expressed in both nuclear and cytoplasm samples of KGN cells. In KGN cells, DLEU1 and miR-146b-5p failed to regulate the expression of each other. However, DLEU1 promoted cell apoptosis and reduced the inhibitory effects of miR-146b-5p on cell apoptosis.ConclusionsDLEU1 is overexpressed in POF and sponges miR-146b-5p to increase KGN cell apoptosis.

【 授权许可】

CC BY